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Springer Lab Manuals

Chicken Egg Yolk Antibodies, Production and Application

IgY-Technology

Editors: Schade, R., Behn, I., Erhard, M., Hlinak, A., Staak, C. (Eds.)

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  • First laboratory manual on this alternative, non-invasive method for producing polyclonal antibodies
  • The authors were involved in the development of the IgY technology, thus they really know the details
  • Step-by-step instructions including all the information needed to use this technique
  • Procedures will work also in less experienced laboratories
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  • ISBN 978-3-662-04488-9
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About this book

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals, especially rabbits. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Since hens can be kept under nearly natural conditions and antibodies be isolated from the collected eggs, this technology has become an interesting alternative to the ,bloody" techniques. For their work on yolk antibodies, the authors of this book received the FISEA prize (International Foundation for the Substitution of Animal Experimentation, Luxembourg) in 1997. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual. These practical instructions are complemented by a short introduction to the hen's humoral immune system and a section on the pros and cons of chicken yolk antibodies compared to those of mammals'serum. Avian antibodies have been shown to be in some applications even more effective than mamalian antibodies, especially when phylogenetically highly conserved antigens have been used for immunisation.

Table of contents (8 chapters)

Table of contents (8 chapters)

Buy this book

eBook $79.99
price for USA in USD
  • ISBN 978-3-662-04488-9
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.99
price for USA in USD
  • ISBN 978-3-662-04490-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chicken Egg Yolk Antibodies, Production and Application
Book Subtitle
IgY-Technology
Editors
  • Rüdiger Schade
  • Ingrid Behn
  • Michael Erhard
  • Andreas Hlinak
  • Christian Staak
Series Title
Springer Lab Manuals
Copyright
2001
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-04488-9
DOI
10.1007/978-3-662-04488-9
Softcover ISBN
978-3-662-04490-2
Edition Number
1
Number of Pages
XII, 255
Number of Illustrations
33 b/w illustrations, 3 illustrations in colour
Topics

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