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Chicken Egg Yolk Antibodies, Production and Application

IgY-Technology

  • Book
  • © 2001

Overview

Editors:
  1. Rüdiger Schade

    1. Institut für Pharmakologie und Toxikologie, Universitätsklinikum Charité der Humboldt Universität zu Berlin, Berlin, Germany

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  2. Ingrid Behn

    1. Pharmazie und Psychologie Institut für Zoologie, Universität Leipzig, Leipzig, Germany

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  3. Michael Erhard

    1. Veterinär-Physiologisches Institut, Universität Leipzig, Leipzig, Germany

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  4. Andreas Hlinak

    1. Institut für Virologie, Freie Universität Berlin, Berlin, Germany

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  5. Christian Staak

    1. Verbraucherschutz und Veterinärmedizin (BgVV), Institut für Veterinärmedizin, FG 505, Bundesinstitut für gesundheitlichen, Berlin, Germany

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  • First laboratory manual on this alternative, non-invasive method for producing polyclonal antibodies
  • The authors were involved in the development of the IgY technology, thus they really know the details
  • Step-by-step instructions including all the information needed to use this technique
  • Procedures will work also in less experienced laboratories

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (8 chapters)

  1. Front Matter

    Pages II-XII
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  2. Short Introduction to Hens’ Humoral Immune System

    • Michael Erhard, Rüdiger Schade
    Pages 1-8

  3. Possibilities of Keeping Laying-Hens to Obtain Antibodies

    • Andreas Hlinak, Rüdiger Schade
    Pages 9-24

  4. Immunisation

    • Christine Schwarzkopf, Christian Staak, Irene Behn, Michael Erhard
    Pages 25-64

  5. Isolation of IgY from Yolk

    • Christian Staak, Christine Schwarzkopf, Ingrid Behn, Undine Hommel, Andreas Hlinak, Rüdiger Schade et al.
    Pages 65-107

  6. Use of Polyclonal Avian Antibodies

    • Ingrid Behn, Undine Hommel, Michael Erhard, Andreas Hlinak, Rüdiger Schade, Christine Schwarzkopf et al.
    Pages 108-210

  7. Pros and Cons of Obtaining Antibodies from Chickens’ Eggs Rather than from Mammals’ Serum

    • Rüdiger Schade, Christine Schwarzkopf, Michael Erhard
    Pages 211-217

  8. IgY-Antibodies, Future Prospects

    • Rüdiger Schade
    Pages 218-222

  9. Appendix

    • Rüdiger Schade
    Pages 223-249

  10. Back Matter

    Pages 251-255
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Keywords

About this book

Polyclonal antibodies, widely used in research and diagnostics, are conventionally isolated from the blood of immunised mammals. The fact that antibodies can also be detected in the yolk of eggs laid by immunised hens, led to the development of the yolk antibody technology as an alternative method less stressful to animals. Hens can be kept under nearly natural conditions and antibodies be isolated from the eggs.
Further, the IgY-technology has the advantage that avian antibodies can be produced against several antigens which do not induce a immune response in mammals. Protocols on how to keep and immunise hens and on the extraction, isolation and use of antibodies from yolk are described in detail in this manual.
For their work on yolk antibodies the authors received the FISEA (International Foundation for the Substitution of Animal Experimentation, Luxembourg) prize in 1997.

Editors and Affiliations

  • Institut für Pharmakologie und Toxikologie, Universitätsklinikum Charité der Humboldt Universität zu Berlin, Berlin, Germany

    Rüdiger Schade

  • Pharmazie und Psychologie Institut für Zoologie, Universität Leipzig, Leipzig, Germany

    Ingrid Behn

  • Veterinär-Physiologisches Institut, Universität Leipzig, Leipzig, Germany

    Michael Erhard

  • Institut für Virologie, Freie Universität Berlin, Berlin, Germany

    Andreas Hlinak

  • Verbraucherschutz und Veterinärmedizin (BgVV), Institut für Veterinärmedizin, FG 505, Bundesinstitut für gesundheitlichen, Berlin, Germany

    Christian Staak

Bibliographic Information

  • Book Title: Chicken Egg Yolk Antibodies, Production and Application

  • Book Subtitle: IgY-Technology

  • Editors: Rüdiger Schade, Ingrid Behn, Michael Erhard, Andreas Hlinak, Christian Staak

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-04488-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2001

  • Softcover ISBN: 978-3-662-04490-2Published: 21 January 2013

  • eBook ISBN: 978-3-662-04488-9Published: 14 November 2014

  • Edition Number: 1

  • Number of Pages: XII, 255

  • Number of Illustrations: 33 b/w illustrations, 3 illustrations in colour

  • Topics: Biotechnology, Immunology, Molecular Medicine, Cell Biology

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