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Antibody Usage in the Lab

  • Book
  • © 1999

Overview

Editors:
  1. Laura Caponi

    1. Department of Clinical Medicine, Clinical Immunology Unit, University of Pisa, Pisa, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Paola Migliorini

    1. Department of Clinical Medicine, Clinical Immunology Unit, University of Pisa, Pisa, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • First lab manual describing the most useful immunological techniques based on antibodies Easy-to-follow step-by-step instructions with troubleshooting guide Practical hints and experimental strategies are given for each protocol
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (7 chapters)

  1. Front Matter

    Pages I-VIII
    Download chapter PDF

  2. Introduction

    • Laura Caponi
    Pages 1-8

  3. ELISA assays

    • Laura Caponi, Paola Migliorini
    Pages 9-31

  4. Immunoblotting

    • Laura Caponi, Paola Migliorini
    Pages 32-46

  5. Immunoprecipitation

    • Paola Migliorini
    Pages 47-60

  6. Immunofluorescence

    • Marina Fabbi, Micaela Tiso
    Pages 61-83

  7. Immunohistochemistry

    • Giancarlo Carbone
    Pages 84-124

  8. Antibody Specificity analyzed by Peptides synthesized on Cellulose Membranes

    • Paolo Rovero, Daniela Lucchesi
    Pages 125-144

  9. Back Matter

    Pages 145-154
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Keywords

About this book

A handy lab manual that permits quick and easy access to the techniques commonly used to analyse antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, and basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.

Editors and Affiliations

  • Department of Clinical Medicine, Clinical Immunology Unit, University of Pisa, Pisa, Italy

    Laura Caponi, Paola Migliorini

Bibliographic Information

  • Book Title: Antibody Usage in the Lab

  • Editors: Laura Caponi, Paola Migliorini

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-03942-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1999

  • Softcover ISBN: 978-3-662-03944-1Published: 29 November 2012

  • eBook ISBN: 978-3-662-03942-7Published: 09 March 2013

  • Edition Number: 1

  • Number of Pages: VIII, 154

  • Number of Illustrations: 19 b/w illustrations

  • Topics: Cell Biology, Biochemistry, general

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