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Engineering of Halogenases towards Synthetic Applications

Increasing the Thermostability and Investigations on a Marine Brominase Bmp5

  • Book
  • © 2017

Overview

Authors:
  1. Hannah Minges

    1. Bielefeld, Germany

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  • Evolution and investigations on halogenases
  • Includes supplementary material: sn.pub/extras

Part of the book series: BestMasters (BEST)

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Table of contents (9 chapters)

  1. Front Matter

    Pages I-XII
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  2. Introduction

    • Hannah Minges
    Pages 13-24

  3. Motivation

    • Hannah Minges
    Pages 25-25

  4. Material and Methods

    • Hannah Minges
    Pages 27-57

  5. Directed evolution of Thal: Results

    • Hannah Minges
    Pages 59-79

  6. Directed evolution of Thal: Discussion

    • Hannah Minges
    Pages 81-87

  7. Establishment of a marine brominase: Results

    • Hannah Minges
    Pages 89-108

  8. Establishment of a marine brominase

    • Hannah Minges
    Pages 109-119

  9. Outlook

    • Hannah Minges
    Pages 121-122

  10. Summary

    • Hannah Minges
    Pages 123-126

  11. Back Matter

    Pages 127-128
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Keywords

About this book

Hannah Minges focuses on the investigation of two different FAD-dependent halogenases in order to analyze and improve their applicability for chemoenzymatic approaches in chemistry. Owing to beneficial features, like high selectivity and benign reaction conditions, nature’s toolkit for halogenation provides several advantages, whereas conventional chemical strategies require hazardous reagents and suffer from low selectivity. Therefore, enzymatic halogenation arises as promising alternative in the synthesis of valuable chemicals. One project focuses on the generation of a thermostable variant of the tryptophan halogenase Thal by means of directed evolution. The second project deals with the investigation of the marine halogenase Bmp5. This enzyme is of synthetic interest because it preferably introduces bromine into phenol compounds, whereas chlorination cannot take place. 

Authors and Affiliations

  • Bielefeld, Germany

    Hannah Minges

About the author

Hannah Minges, a Ph.D student at Bielefeld University, works on the optimization of tryptophan halogenases by means of directed evolution.

Bibliographic Information

  • Book Title: Engineering of Halogenases towards Synthetic Applications

  • Book Subtitle: Increasing the Thermostability and Investigations on a Marine Brominase Bmp5

  • Authors: Hannah Minges

  • Series Title: BestMasters

  • DOI: https://doi.org/10.1007/978-3-658-18410-0

  • Publisher: Springer Spektrum Wiesbaden

  • eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)

  • Copyright Information: Springer Fachmedien Wiesbaden GmbH 2017

  • Softcover ISBN: 978-3-658-18409-4Published: 23 May 2017

  • eBook ISBN: 978-3-658-18410-0Published: 11 May 2017

  • Series ISSN: 2625-3577

  • Series E-ISSN: 2625-3615

  • Edition Number: 1

  • Number of Pages: XII, 116

  • Number of Illustrations: 37 b/w illustrations, 9 illustrations in colour

  • Topics: Organic Chemistry, Catalysis, Green Chemistry

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