Initial Rate Enzyme Kinetics

1. Front Matter

   Pages I-X

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2. Nomenclature, Definitions, and Evolution of the Kinetic Mechanism

   • Herbert J. Fromm

   Pages 1-21

3. Derivation of Initial Velocity Rate Equations

   • Herbert J. Fromm

   Pages 22-40

4. Experimental Protocol and Plotting of Kinetic Data

   • Herbert J. Fromm

   Pages 41-82

5. Use of Competitive Substrate Analogs and Alternative Substrates for Studying Kinetic Mechanisms

   • Herbert J. Fromm

   Pages 83-120

6. Product, Substrate, and Alternative Substrate Inhibition

   • Herbert J. Fromm

   Pages 121-160

7. Isotope Exchange

   • Herbert J. Fromm

   Pages 161-185

8. Isomerization Mechanisms and the Φ and Haldane Relationships

   • Herbert J. Fromm

   Pages 186-200

9. The Effect of Temperature and pH on Enzyme Activity

   • Herbert J. Fromm

   Pages 201-235

10. Cooperativity and Allostery

    • Herbert J. Fromm

    Pages 236-280

11. Back Matter

    Pages 281-324

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Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem­ ical literature reveals the increasing popularity of enzyme ki­ netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math­ ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis­ ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.

• Enzym
• Nucleotide
• Termination
• amino acid
• biochemistry
• chemistry
• development
• enzyme
• enzyme kinetics
• enzymes
• kinetics
• protein
• temperature

• Biochemistry and Biophysics Department, Iowa State University, Ames, USA

  Herbert J. Fromm

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