Current Topics in Microbiology and Immunology

The Molecular Biology of Bacterial Virus Systems

Editors: Hobom, G., Rott, Rudolf (Eds.)

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The fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3' -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag­ ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5' -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin­ dromic nucleotide sequence at the 3' -end that allows the formation of a hairpin structure which provides the needed free 3'-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli­ cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specific nicking of one of the strands of a circular double-stranded DNA, producing a 3'-OH group available for elongation (KORNBERG 1980).

Table of contents (4 chapters)

Table of contents (4 chapters)

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-3-642-73115-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $109.00
price for USA in USD
  • ISBN 978-3-642-73117-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Molecular Biology of Bacterial Virus Systems
Editors
  • G. Hobom
  • Rudolf Rott
Series Title
Current Topics in Microbiology and Immunology
Series Volume
136
Copyright
1988
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-73115-0
DOI
10.1007/978-3-642-73115-0
Softcover ISBN
978-3-642-73117-4
Series ISSN
0070-217X
Edition Number
1
Number of Pages
VII, 90
Topics