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Ultraviolet Spectroscopy of Proteins

Authors: Demchenko, Alexander P.

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  • ISBN 978-3-642-70847-3
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About this book

The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Introduction

    Pages 1-4

    Demchenko, Professor Dr. Alexander P.

  • Spectroscopic Properties of Protein Chromophores

    Pages 5-26

    Demchenko, Professor Dr. Alexander P.

  • Display of Intramolecular and Intermolecular Interactions in Electronic Spectra of Amino Acids and Proteins

    Pages 27-63

    Demchenko, Professor Dr. Alexander P.

  • Difference Spectra of Proteins

    Pages 64-90

    Demchenko, Professor Dr. Alexander P.

  • Thermal Perturbation Difference Spectroscopy and Temperature-Dependent Conformational Transitions of Proteins

    Pages 91-120

    Demchenko, Professor Dr. Alexander P.

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-3-642-70847-3
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.99
price for USA in USD
  • ISBN 978-3-642-70849-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Ultraviolet Spectroscopy of Proteins
Authors
Copyright
1986
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-70847-3
DOI
10.1007/978-3-642-70847-3
Softcover ISBN
978-3-642-70849-7
Edition Number
1
Number of Pages
XI, 312
Additional Information
Revised and enlarged translation of the original Russian edition published by Naukova Dumka, Kiev 1981
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