The Physiological Properties of Plant Protoplasts

1. Front Matter

   Pages I-XIII

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2. Introduction: The Use of Plant Protoplasts in Physiological Research

   • P. E. Pilet

   Pages 1-5

3. Applications of Protoplast Technology

   • E. C. Cocking

   Pages 6-11

4. Properties of Some Enzymes Used for Protoplast Isolation

   • P. J. Fitzsimons, J. D. B. Weyers

   Pages 12-23

5. Isolation of Maize Protoplasts from the Root Cap and Apex

   • P. E. Pilet, H. Henry, C. Jollès

   Pages 24-28

6. Plant Protoplast Viability

   • C. H. Bornman, J. F. Bornman

   Pages 29-36

7. Isolation of Plasma Membrane from Ryegrass (Lolium multiflorum) Endosperm Protoplasts

   • A. Schibeci

   Pages 37-44

8. The Use of Protoplasts in the Study of Coated Vesicles

   • B. G. Mersey, L. R. Griffing, L. C. Fowke

   Pages 45-53

9. Membrane Transport in Protoplasts

   • P. Morris

   Pages 54-67

10. The Binding of Anion Transport Inhibitors on the Plasmalemma Isolated from Corn Root Protoplasts

    • W. Lin

    Pages 68-75

11. Intracellular Transport of Metabolites in Protoplasts: Transport Between Cytosol and Vacuole

    • Th. Boller

    Pages 76-86

12. Compartmentation of Metabolite Pools in Protoplasts: Chloroplasts, Mitochondria, Cytosol/Vacuole

    • R. Hampp

    Pages 87-98

13. Protoplast Evacuolation

    • R. J. Griesbach, R. H. Lawson

    Pages 99-104

14. Protoplasts in Studies of Vacuolar Storage Compounds

    • G. Alibert, A. M. Boudet, H. Canut, P. Rataboul

    Pages 105-115

15. Distribution of Saccharides Between Cytoplasm and Vacuole in Protoplasts

    • F. Keller, P. Matile, A. Wiemken

    Pages 116-121

16. Anthocyanin-Containing Vacuoles Isolated from Protoplasts of Daucus carota Cell Cultures

    • W. Hopp, W. Hinderer, M. Petersen, H. U. Seitz

    Pages 122-132

17. Vacuolar pH Variability in a Protoplast Population

    • P. Manigault

    Pages 133-142

18. Mitotic Cycle of Mesophyll Protoplasts

    • Y. Meyer

    Pages 143-151

19. The Use of Guard Cell Protoplasts to Study Stomatal Physiology

    • J. D. B. Weyers, P. J. Fitzsimons

    Pages 152-161

20. Regulation of Volume Changes in Guard Cell Protoplasts

    • H. Schnabl

    Pages 162-170

The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro­ vide a subject that would be of scientific, methodological and epistemologi­ cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.

• Pflanzen
• Properties
• Protoplast
• Protoplasts
• physiology

• Institut de Biologie et de Physiologie Végétales, Bâtiment de Biologie de l’Université, Lausanne, Switzerland

  Paul-Emile Pilet

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