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Proceedings in Life Sciences

Hormonally Defined Media

A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982

Editors: Fischer, Gabriele, Wieser, R. J. (Eds.)

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About this book

Until some years ago serum or crude tissue extracts were used pre­ dominantly or exclusively as media supplements for the cultivation of cells. However, during this time evidence accumulated that these sup­ plements could not provide in an optimal way most of the cultivated cells with all factors necessary for their survival, their prolifer­ ation and/or differentiation. Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re­ gard to survival, proliferation and differentiation of cells in cul­ ture. It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti­ vate those cells which could not survive in serum-supplemented media alone. Under normal conditions in an organism, however, only a small population of cells has direct contact with serum.

Table of contents (42 chapters)

  • Divining the Role of Serum in Cell Culture

    Sato, Gordon H.

    Pages 2-3

  • Reducing the Requirement for Serum Supplements in High Yield Microcarrier Cell Culture

    Clark, Julian

    Pages 6-15

  • Growth of Normal Human Cells in Defined Media

    Ham, Richard G.

    Pages 16-30

  • Investigations on the Cultivation of Insect Cell Lines in Serum-Free Media

    Miltenburger, H. G.

    Pages 31-43

  • Different Signalling Systems Control the Initiation of DNA Replication in Cultured Animal Cells

    Jimenez de Asua, Luis (et al.)

    Pages 46-56

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-3-642-69290-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $159.00
price for USA in USD
  • ISBN 978-3-642-69292-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hormonally Defined Media
Book Subtitle
A Tool in Cell Biology Lectures and Posters Presented at the First European Conference on Serum-Free Cell Culture Heidelberg, October 7–9, 1982
Editors
  • Gabriele Fischer
  • R. J. Wieser
Series Title
Proceedings in Life Sciences
Copyright
1983
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-69290-1
DOI
10.1007/978-3-642-69290-1
Softcover ISBN
978-3-642-69292-5
Series ISSN
0172-6625
Edition Number
1
Number of Pages
XIV, 458
Topics