New Developments in Diagnostic Virology

1. Front Matter

   Pages I-XII

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2. Sense and Nonsense in Viral Diagnosis — Past, Present, and Future

   • Ken McIntosh

   Pages 1-6

3. Immune Complexes in Viral Infection

   • Paolo Casali, Michael B. A. Oldstone

   Pages 7-48

4. Immunoscintigraphy for the Detection of Human Carcinoma After Injection of Radiolabeled Monoclonal Anti-Carcino-embryonic Antigen Antibodies

   • Jean-Pierre Mach, Franz Buchegger, Michel Forni, Jörg Ritschard, Stephan Carrel, Rudolf Egley et al.

   Pages 49-55

5. Automated Systems in Viral Diagnosis

   • John L. Sever

   Pages 57-75

6. Data Processing in Clinical Virology

   • Karl-Otto Habermehl

   Pages 77-91

7. Enzyme Immunoassays and Related Techniques: Development and Limitations

   • Stratis Avrameas

   Pages 93-99

8. Detection of Antiviral IgM Antibodies and its Problems — A Review

   • Olli Meurman

   Pages 101-131

9. Detection of Viral Antigens by Time-Resolved Fluoroimmunoassay

   • Pekka Halonen, Olli Meurman, Timo Lövgren, Ikka Hemmilä, Erkkl Soini

   Pages 133-146

10. Uses and Abuses of Diagnostic Electron Microscopy

    • June D. Almeida

    Pages 147-158

11. The Use of Staphylococcal Protein A in Diagnostic Virology

    • Douglas D. Richman

    Pages 159-176

12. Use of Monoclonal Antibodies for Viral Diagnosis

    • Robert H. Yolken

    Pages 177-195

13. High-Resolution Protein Separation and Identification Methods Applicable to Virology

    • Norman G. Anderson

    Pages 197-217

14. Electrofocusing Structural and Induced Proteins of Aphthovirus

    • Andrew M. Q. King, David McCahon, John W. I. Newman, J. R. Crowther, William C. Carpenter

    Pages 219-233

15. A Sensitive Assay for Detection of Deoxythymidine Kinase and its Application to Herpesvirus Diagnosis

    • J. Simon Gronowitz, Claes F. R. Källander

    Pages 235-245

16. The Characterization of Influenza A Viruses by Carbohydrate Analysis

    • Hans-Dieter Klenk, Walter Keil, Heiner Niemann, Rudolf Geyer, Ralf T. Schwarz

    Pages 247-257

17. The Application of RNA Fingerprinting and Sequencing to Viral Diagnosis

    • David H. L. Bishop

    Pages 259-271

18. Restriction Endonuclease Patterns of Herpes Simplex Virus DNA: Application to Diagnosis and Molecular Epidemiology

    • Bernard Roizman, Mauro Tognon

    Pages 273-286

19. Nucleic Acid Hybridization for the Detection of Viral Genomes

    • Georg W. Bornkamm, Claude Desgranges, Lutz Gissmann

    Pages 287-298

20. The Use of Molecular Hybridization for Demonstration of Adenoviruses in Human Stools

    • Per Stålhandske, Timo Hyypiä, Håkan Gadler, Pekka Halonen, Ulf Pettersson

    Pages 299-306

The contributions to this book derived from the Seventh Munich Symposium on Microbiology on June 3 and 4, 1981, which was organized by the WHO Centre for Collection and Evaluation of Data on Comparative Virology at the Institute of Medical Microbiology, Infectious and Epidemic Diseases, University of Munich, Federal Repub­ lic of Germany. One of our principal purposes was to establish a forum at which the comparative aspects of questions of current interest in the field of medical virology could be discussed. In addition to the presentation of recent fmdings in microbiology, our overall aim was to crystallize trends and indicate new directions for future research activities. This book is a topical review of "New Horizons in Diagnostic Virology." Every­ one interested in virology is aware of the tremendous progress made in viral diagnostic techniques during recent years and the growing importance of viral diagnosis in human and veterinary medicine. There is yet another step that diagnostic virology has to take: the introduction on a routine basis of methods of molecular biology into the viral diagnostic laboratory. The application of monoclonal antibodies and techniques for the chemical and biological identification of proteins, carbohydrates, and enzymes are discussed, as is the introduction of techniques for the characterization of nucleic acids in viral diag­ nosis.

• antibody
• diseases
• medical microbiology
• microbiology
• molecular biology
• protein
• proteins
• virology

• Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, München 22, Germany

  Peter A. Bachmann

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