Current Topics in Microbiology and Immunology

T Cell Hybridomas

A Workshop at the Basel Institute for Immunology

Editors: Boehmer, H.v., Haas, W., Köhler, G., Melchers, F., Zeuthen, J. (Eds.)

Free Preview

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-3-642-68586-6
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $159.00
price for USA in USD
  • ISBN 978-3-642-68588-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

For more than ten years cell fusion techniques have been applied in studies on various lymphocyte functions. Ig expression was first studied in hybrids obtained by fusing myeloma cells with fibroblasts (1) or lymphomas (2), both of which do not produce Ig, and with Ig­ producing myelomas (3) or human blood lymphocytes (4). Kohler and Milstein (5) fused a myeloma with spleen cells from immunized mice. Up to 10% of the hybrids obtained secreted antibodies specific for the immunizing antigen. This suggested that plasma cells preferenti­ ally fused with the myeloma cells, a finding which was of enormous practical value. It was found that both Band T lymphocytes could be fused with the T cell tumor BW5147, which is however not permissive for Ig synthesis (6). A very large number of T cell hybridomas were generated by fusing BW5147 with cell populations containing in vivo or in vitro activated cells (7). The hybrids showed no specific T cell functions and binding assays for T cell receptors were not available. In particular, no hybrids were obtained which expreS1ed specific cytolytic activity that could be tested in short-term Cr­ release assays (8). However, the frustrations expressed about these failures, published in January, 1978 (9), were relieved by Taniguchi and Miller's publication a few months later of T cell hybridomas producing antigen-specific suppressor factors (10). Unfortunately, their hybrids rapidly lost factor production.

Table of contents (28 chapters)

Table of contents (28 chapters)
  • Lectin-Dependent Cytolytic and Cytolymic T Helper Clones and Hybridomas

    Fischer Lindahl, K. (et al.)

    Pages 1-10

  • Cytotoxic T Cell Hybridomas: Generation and Characterization

    Eshhar, Z. (et al.)

    Pages 11-18

  • Definition of Function-Related Isotypic Markers on T Cells

    Culbert, E. J. (et al.)

    Pages 19-32

  • An Antigen-Specific Suppressor T Cell Factor Controlled by Two Genes in the Immunoglobulin Heavy Chain Linkage Group and in the I-J Subregion of the H-2 Complex

    Taniguchi, M. (et al.)

    Pages 33-41

  • Structural and Functional Studies on Antigen-Specific Suppressor Factors from T Cells and T Cell Hybrids

    Taussig, M. J. (et al.)

    Pages 43-52

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-3-642-68586-6
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $159.00
price for USA in USD
  • ISBN 978-3-642-68588-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
T Cell Hybridomas
Book Subtitle
A Workshop at the Basel Institute for Immunology
Editors
  • H.v. Boehmer
  • W. Haas
  • G. Köhler
  • F. Melchers
  • J. Zeuthen
Series Title
Current Topics in Microbiology and Immunology
Series Volume
100
Copyright
1982
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-68586-6
DOI
10.1007/978-3-642-68586-6
Softcover ISBN
978-3-642-68588-0
Series ISSN
0070-217X
Edition Number
1
Number of Pages
XII, 264
Topics