Dynamic Morphology of Leukemia Cells

A Comparative Study by Scanning Electron Microscopy and Microcinematography

Authors: Felix, H., Haemmerli, G., Sträuli, P.

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About this book

Dynamic Morphology is the attempt to correlate surface architec­ ture and shape of fixed cells, as visualized by scanning electron microscopy (SEM), with the behavior of living cells, recorded by microcinematography (MCM). If SEM and MCM are used concurrently for the analysis of cell populations, a dynamic inter­ pretation of SEM photographs is only valid if the experimental conditions are identical for the two techniques. This is achieved by allowing the cells to settle on a glass surface where they remain long enough to perform their various activities under conditions identical for both techniques (for technical details see Methodology). The analysis of a population necessitates the study of a large number of cells. This prerequisite is met by operating the scanning electron micro­ scope at low levels of magnification, and by using culture chambers for cinematography. It can be argued that the examina­ tion of attached cells excludes a complete SEM survey of a population, as cells not adhering from the outset or becoming detached during the different preparatory steps are lost. For this, cinematography proved to be a reliable control: All cell types recognized in time-lapse films were also seen in scanning electron (SE) micrographs. Another, and more general, objection to a dynamic interpretation concerns the artificiality of cellular behavior on glass. This is true, but does not invalidate compara­ tive studies making use of this substrate.

Table of contents (5 chapters)

Table of contents (5 chapters)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-3-642-66794-7
  • Digitally watermarked, DRM-free
  • Included format:
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.00
price for USA in USD
  • ISBN 978-3-642-66796-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Dynamic Morphology of Leukemia Cells
Book Subtitle
A Comparative Study by Scanning Electron Microscopy and Microcinematography
Authors
Copyright
1978
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin · Heidelberg
eBook ISBN
978-3-642-66794-7
DOI
10.1007/978-3-642-66794-7
Softcover ISBN
978-3-642-66796-1
Edition Number
1
Number of Pages
XIV, 194
Number of Illustrations
111 b/w illustrations
Topics