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Rapid Cycle Real-Time PCR — Methods and Applications

Genetics and Oncology

Editors: Dietmaier, W., Wittwer, Carl, Sivasubramanian, N. (Eds.)

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eBook $84.99
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  • ISBN 978-3-642-59397-0
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Softcover $109.00
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  • ISBN 978-3-642-63965-4
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About this book

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier!

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Introduction for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications

    Pages 1-2

    Dietmaier, Dr. Wolfgang (et al.)

  • Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument

    Pages 5-14

    Zhu, Xi (et al.)

  • Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards

    Pages 15-25

    Kielar, Danuta (et al.)

  • Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene

    Pages 27-33

    Pieri-Balandraud, Nathalie (et al.)

  • Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes

    Pages 35-46

    Crockett, Andrew O.

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-3-642-59397-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $109.00
price for USA in USD
  • ISBN 978-3-642-63965-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rapid Cycle Real-Time PCR — Methods and Applications
Book Subtitle
Genetics and Oncology
Editors
  • W. Dietmaier
  • Carl Wittwer
  • N. Sivasubramanian
Copyright
2002
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-59397-0
DOI
10.1007/978-3-642-59397-0
Softcover ISBN
978-3-642-63965-4
Edition Number
1
Number of Pages
VIII, 205
Number of Illustrations
11 b/w illustrations, 528 illustrations in colour
Topics