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Cell-Free Protein Expression

Editors: Swartz, James R. (Ed.)

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  • ISBN 978-3-642-59337-6
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Softcover $139.99
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  • ISBN 978-3-642-63939-5
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About this book

 

Cell-free protein synthesis is coming of age! Motivated by an

escalating need for efficient protein synthesis and empowered by

readily accessible cell-free protein synthesis kits, the technology

is expanding both in the range of feasible proteins and in the ways

that proteins can be labeled and modified. This volume follows

"Cell-Free Translation Systems", edited by Professor Alexander S.

Spirin in 2002.

Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability.

In this volume, we show that proteins now can be efficiently produced using PCR products as DNA

templates and that even membrane proteins and proteins with multiple

disulfide proteins are obtained at high yields. Many additional

advances are also presented. It is an exciting time for protein

synthesis technology.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Generation of Linear Expression Elements by PCR

    Pages 3-7

    Nemetz, Dr. Cordula

  • Rapid Protein Engineering by Expression-PCR

    Pages 9-13

    Nemetz, Dr. Cordula (et al.)

  • Expression-PCR: from Gene Pools to Purified Proteins Within 1 Day

    Pages 15-23

    Merk, H. (et al.)

  • High-Throughput Expression PCR Used to Systematically Investigate Regulation of Translation Initiation in an E. coli Cell-Free Expression System

    Pages 25-34

    Watzele, Dr. Manfred (et al.)

  • Reduction of Primer-Dimer Formation during Generation of Expression Fragments by PCR

    Pages 35-39

    Gräntzdörffer, Dr. A. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-3-642-59337-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.99
price for USA in USD
  • ISBN 978-3-642-63939-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Cell-Free Protein Expression
Editors
  • James R. Swartz
Copyright
2003
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-59337-6
DOI
10.1007/978-3-642-59337-6
Softcover ISBN
978-3-642-63939-5
Edition Number
1
Number of Pages
X, 230
Number of Illustrations
40 illustrations in colour
Topics