Rapid Cycle Real-Time PCR — Methods and Applications

Quantification

Editors: Wittwer, Carl, Hahn, Meinhard, Kaul, Karen (Eds.)

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  • ISBN 978-3-642-18840-4
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About this book

Rapid Cycle Real-Time PCR is a powerful technique for nucleic

acid quantification and analysis that takes less than 30 minutes

to complete. Fluorescence is automatically monitored

each cycle and the amount of template quantified by advanced

analytical methods, such as the second derivative maximum method.

Immediately following rapid cycle PCR, melting curve analysis is

performed to verify product purity with SYBR Green I and/or

genotype with fluorescently-labeled hybrdidization probes

(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often

cited as the most versatile, efficient method for nucleic acid

quantification in research and climical studies. Molecular analysis

has never been easier!

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Housekeeping Genes: A Gold Standard?

    Pages 3-10

    Bakker, Onno (et al.)

  • The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia

    Pages 11-20

    Weisser, Martin (et al.)

  • Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach

    Pages 21-29

    Ramakers, Christian (et al.)

  • Measuring Genome Sizes by Absolute Quantification

    Pages 31-41

    Wilhelm, Jochen (et al.)

  • Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I

    Pages 45-51

    Neubauer, Nicole

Buy this book

eBook $59.99
price for USA in USD (gross)
  • ISBN 978-3-642-18840-4
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $79.99
price for USA in USD
  • ISBN 978-3-642-62317-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rapid Cycle Real-Time PCR — Methods and Applications
Book Subtitle
Quantification
Editors
  • Carl Wittwer
  • Meinhard Hahn
  • Karen Kaul
Copyright
2004
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-18840-4
DOI
10.1007/978-3-642-18840-4
Softcover ISBN
978-3-642-62317-2
Edition Number
1
Number of Pages
VII, 223
Number of Illustrations
15 b/w illustrations, 511 illustrations in colour
Topics