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Rapid Cycle Real-Time PCR

Methods and Applications

Editors: Meuer, S., Wittwer, Carl, Nakagawara, K. (Eds.)

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  • ISBN 978-3-642-59524-0
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About this book

Rapid Cycle Real-Time PCR allows quantification and sequence analysis in less than 30 minutes. This powerful technique can be applied to any nucleic acid for rapid testing in microbiology, genetics, and oncology. Fluorescence analysis provides convenient determination of product of probe melting temperatures that allows product identification, even down to a single base.

Table of contents (43 chapters)

Table of contents (43 chapters)
  • Rapid Cycle Real-Time PCR: Methods and Applications

    Pages 1-8

    Wittwer, Carl

  • Mutation Detection by Fluorescent Hybridization Probe Melting Curves

    Pages 11-19

    Bernard, Philip S. (et al.)

  • Quantification on the LightCycler

    Pages 21-34

    Rasmussen, Randy

  • Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis

    Pages 35-41

    Landt, Olfert

  • Using the Nearest Neighbor Model for the Estimation of Matched and Mismatched Hybridization Probe Melting Points and Selection of Optimal Probes on the LightCycler

    Pages 43-56

    Ahsen, Nicolas (et al.)

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-3-642-59524-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Rapid Cycle Real-Time PCR
Book Subtitle
Methods and Applications
Editors
  • S. Meuer
  • Carl Wittwer
  • K. Nakagawara
Copyright
2001
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-59524-0
DOI
10.1007/978-3-642-59524-0
Edition Number
1
Number of Pages
XI, 408
Number of Illustrations
72 b/w illustrations, 70 illustrations in colour
Topics