Plant Molecular Biology — A Laboratory Manual

1. Front Matter

   Pages I-XXIX

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2. Basic Molecular Techniques

   1. Front Matter

      Pages 1-1

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   2. Genomic DNA Isolation, Southern Blotting and Hybridization

      • S. Wilkie, M. S. Clark, P. Leroy, M. Merlino, S. Nègre, J. C. Caissard et al.

      Pages 3-53

   3. Cloning from Genomic DNA and Production of Libraries

      • A. Guevara-Garcia, L. Herrera-Estrella, G. Olmedo-Alvarez, M. S. Clark, G. Elgar, C.-N. Liu et al.

      Pages 54-153

   4. Extraction of RNA, Cloning and Subtractive Hybridization

      • P. Lessard, V. Decroocq, M. Thomas, H. C. C. Foote, G. Brady, G. J. Thorlby et al.

      Pages 154-220

   5. Characterization of Clones

      • J. R. Clark, T. Pehu

      Pages 221-277

3. Characterization of Plant DNA

   1. Front Matter

      Pages 279-279

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   2. Organelle DNA Isolation

      • M. Landgren, K. Glimelius

      Pages 281-304

   3. RAPD Analysis: Use for Genome Characterization, Tagging Traits and Mapping

      • R. Waugh

      Pages 305-333

   4. RFLP Mapping of Plant Nuclear Genomes: Planning of Experiments, Linkage Map Construction, and QTL Mapping

      • J. H. van den Berg, S. D. Chasalow, R. Waugh

      Pages 334-396

4. Genetic Engineering: Methodology and Analysis

   1. Front Matter

      Pages 397-397

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   2. Plant Gene Transfer

      • R. H. Potter, M. G. K. Jones

      Pages 399-426

   3. Molecular Characterization of Transformed Plants

      • J. F. Topping, K. Lindsey

      Pages 427-442

   4. Molecular Characterization of Somatic Hybrids

      • Y.-S. Xu, E. Pehu

      Pages 443-460

   5. Cytological Characterization of Transformed Plants: Mapping of Low-Copy and Repetitive DNA Sequences by Fluorescent In Situ Hybridization (FISH)

      • I. J. Leitch, A. Y. Kenton, A. S. Parokonny, M. D. Bennett

      Pages 461-485

   6. Cytological Characterization of Somatic Hybrids: Detection of Genome Origin by Genomic In Situ Hybridization (GISH)

      • A. Y. Kenton, A. S. Parokonny, I. J. Leitch, M. D. Bennett

      Pages 486-511

   7. Addendum to Chapters 11 and 12 In Situ Hybridization in Plant Species with Small Chromosomes

      • N. Lapitan

      Pages 512-519

5. Back Matter

   Pages 521-529

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The aim of this manual is to encompass a broad range of the latest plant molecular biology techniques. However, it is acknowledged that any manual will be read (and hopefully) used by a wide range of people with different levels of experience. Hence the remit of the manual was widened to include a full range of basic molecular tech­ niques, so that novices do not have to consult several texts to enable the execution of each major experiment. The manual is divided into three main parts: Part I: Basic Molecular Techniques The raison d'etre behind this part is to provide a background knowledge of molecular techniques, but also to reduce duplication in later chapters (this is particularly true of the methods contained in Chap. 1). All authors provided very detailed methods and often forgot that so me of these would be covered earlier. A particular favourite was DNA extraction methods, wherc everyone managed to provide a slightly different variant! My view was that it is far less confusing for the reader to be presented with one standard protocol and accom­ panying troubleshooting tips, than to read a different version in each chapter. In this way the basic techniques are addressed more in depth (and my apologies to all authors for judicious use of the delete key!). RNA methodology is covered in Chapter 3. This proceeds from the fundamentals of extraction, northern blotting etc. , to cDNA libraries.

• DNA
• PCR
• RNA
• YAC
• biology
• cytogenetics
• gene
• gene transfer
• genes
• genetic engineering
• genetics
• molecular biology
• plant
• plant genetics
• plants

"Overall, this manual deserves to be highly recommended due, in particular, to the clarity of writing. ...this manual will be purchased and used by many in this rapidly expanding area of research." Heredity
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist

• Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s Hospital, University of Cambridge, Cambridge, UK

  Melody S. Clark

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