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RNP Particles, Splicing and Autoimmune Diseases

  • Book
  • © 1998

Overview

Editors:
  1. Johannes Schenkel

    1. II. Physiologisches Institut, Universität Heidelberg, Heidelberg, Germany

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  • A practical tool for anyone working at the lab bench
  • Easy to follow step-by-step protocols
  • Background information and full practical details

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (8 chapters)

  1. Front Matter

    Pages I-X
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  2. Isolation and Immunochemical Characterization of hnRNP Particles

    • Frank Jung, Constantin E. Sekeris, Johannes Schenkel
    Pages 1-28

  3. The InRNP Particle — A Naturally Assembled Complex of Pre-mRNA and Splicing Factors

    • Ruth Sperling, Joseph Sperling
    Pages 29-47

  4. Intrinsic Fluorescence Techniques for Studies on Protein-Protein and Protein-RNA Interactions in RNP Particles

    • Karl Otto Greulich
    Pages 48-71

  5. Procedures for Three-Dimensional Reconstruction from Thin Sections with Electron Tomography

    • Ulf Skoglund, Lars-Göran Öfverstedt, Bertil Daneholt
    Pages 72-94

  6. Purification and Electron Microscopy of Spliceosomal snRNPs

    • Berthold Kastner
    Pages 95-140

  7. Detection of Autoantibodies to Extractable Cellular Antigens

    • Panayiotis G. Vlachoyiannopoulos
    Pages 141-154

  8. Methods in Immunolocalization of Autoantigens

    • Martin Blüthner
    Pages 155-183

  9. In Vitro Splicing of Pre-mRNA in HeLa Extracts

    • Johannes Schenkel, Frank Jung, Apostolia Guialis, Angela Krämer
    Pages 184-210

  10. Back Matter

    Pages 211-225
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Keywords

About this book

The maturation of the precursors of mRNA to a functional transcript in eukaryotic cells is a complex, multistep process. It includes the formation of RNP complexes and the splicing procedure. Also, the presence of snRNA packaged as snRNP particles plays an important role in this process. In addition, snRNPs are involved in various autoimmune diseases in which autoantibodies are directed against components of these particles.
Relevant techniques to investigate RNA processing, splicing, protein-RNA complex formation and protein-RNA interactions as well as the use of autoantibodies in basic and clinical research are presented in this manual.

Editors and Affiliations

  • II. Physiologisches Institut, Universität Heidelberg, Heidelberg, Germany

    Johannes Schenkel

Bibliographic Information

  • Book Title: RNP Particles, Splicing and Autoimmune Diseases

  • Editors: Johannes Schenkel

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-80356-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-642-48975-4Published: 18 July 2012

  • eBook ISBN: 978-3-642-80356-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: X, 225

  • Number of Illustrations: 90 b/w illustrations

  • Topics: Cell Biology, Biochemistry, general, Molecular Medicine

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