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  • © 1995

Gene Transfer to Plants

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (33 chapters)

  1. Front Matter

    Pages I-XXII
  2. Agrobacterium tumefaciens-Mediated Gene Transfer

    1. Front Matter

      Pages 1-4
  3. Agrobacterium rhizogenes-Mediated Gene Transfer

    1. Front Matter

      Pages 39-44
  4. Biolistic Plant Transformation

    1. Front Matter

      Pages 113-117
    2. Protocol

      1. Transient Gene Expression Studies with the Biolistic System
        • D. A. Russell, M. E. Fromm
        Pages 118-126
      2. Maize Transformation by Microprojectile Bombardment of Immature Embryos
        • E. Dunder, J. Dawson, J. Suttie, G. Pace
        Pages 127-138

About this book

Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics.
Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Also included are protocols of the standard molecular techniques for the analysis of transgenic plants as well as a section on biosafety considerations and regulations.

Reviews

"The protocols are described as step-by-step procedures in such a way that everybody with some basic knowledge in biological experimentation will be able to repeat the experiments without much further reading."
Journal of Plant Physiology
"...well worth the investment."
Plant Growth Regulation

Editors and Affiliations

  • Swiss Federal Institute of Technology, Institute of Plant Sciences, ETH Zürich, Zürich, Switzerland

    Ingo Potrykus, German Spangenberg

Bibliographic Information

  • Book Title: Gene Transfer to Plants

  • Editors: Ingo Potrykus, German Spangenberg

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-79247-2

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-642-48967-9Published: 03 October 2013

  • eBook ISBN: 978-3-642-79247-2Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XXII, 362

  • Number of Illustrations: 66 b/w illustrations, 16 illustrations in colour

  • Topics: Cell Biology, Biotechnology, Plant Sciences, Food Science, Agriculture, Forestry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access