Editors:
- First book dealing with all aspects of restriction endonucleases
- Includes supplementary material: sn.pub/extras
Part of the book series: Nucleic Acids and Molecular Biology (NUCLEIC, volume 14)
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Table of contents (16 chapters)
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Front Matter
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Back Matter
About this book
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Editors and Affiliations
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Institute for Biochemistry, Justus-Liebig-University, Giessen, Germany
Alfred M. Pingoud
Bibliographic Information
Book Title: Restriction Endonucleases
Editors: Alfred M. Pingoud
Series Title: Nucleic Acids and Molecular Biology
DOI: https://doi.org/10.1007/978-3-642-18851-0
Publisher: Springer Berlin, Heidelberg
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eBook Packages: Springer Book Archive
Copyright Information: Springer-Verlag Berlin Heidelberg 2004
Hardcover ISBN: 978-3-540-20502-9Published: 19 February 2004
Softcover ISBN: 978-3-642-62324-0Published: 21 September 2012
eBook ISBN: 978-3-642-18851-0Published: 06 December 2012
Series ISSN: 0933-1891
Series E-ISSN: 1869-2486
Edition Number: 1
Number of Pages: XXVI, 443
Topics: Biochemistry, general, Biotechnology, Cell Biology, Microbiology