Skip to main content
SpringerLink
Log in

Electrotransformation of Bacteria

  • Book
  • © 2000

Overview

Editors:
  1. Natalie Eynard

    1. Institut de Pharmacologie et Biologie Structurale, CNRS UPR 9062, Toulouse cédex, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Justin Teissié

    1. Institut de Pharmacologie et Biologie Structurale, CNRS UPR 9062, Toulouse cédex, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Protocols on the transformation of about 40 bacterial strains of biotechnolgical or medical relevance
  • Easy-to-follow step-by-step instructions for immediate use at the lab bench
  • Each experiment shows the uniform clear structure and presentation of a Springer Lab Manual

Part of the book series: Springer Lab Manuals (SLM)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (33 chapters)

  1. Front Matter

    Pages I-XI
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. General Principles of Bacteria Electrotransformation: Key Steps

      • Natalie Eynard, Justin Teissié
      Pages 3-22

    3. A Critical Introduction to the Technology of Bacterial Electrotransformation

      • Justin Teissié
      Pages 23-34

    4. Electrotransformation of Escherichia coli

      • Natalie Eynard, Justin Teissié
      Pages 35-41

    5. Transformation of Bacillus subtilis PB1424 by Electroporation

      • Patrizia Brigidi, Maddalena Rossi, Diego Matteuzzi
      Pages 42-46

  3. Biotechnology and Food Technology

    1. Front Matter

      Pages 47-47
      Download chapter PDF

    2. Clostridium in Biotechnology and Food Technology

      • Hans P. Blaschek, Joseph Formanek, C. K. Chen
      Pages 49-55

    3. Electrotransformation of Lactococcus lactis

      • Pascal Le Bourgeois, Philippe Langella, Paul Ritzenthaler
      Pages 56-65

    4. Electrotransformation of Salmonella typhimurium

      • Natalie Eynard
      Pages 66-71

    5. Electroporation of bifidobacteria

      • Maddalena Rossi, Patrizia Brigidi, Diego Matteuzzi
      Pages 72-77

    6. Electrotransformation of Listeria species

      • Janet E. Alexander
      Pages 78-87

    7. Transformation of Methylobacterium extorquens with a Broad-Host-Range Plasmid by Electroporation

      • Shunsaku Ueda, Tsuneo Yamane
      Pages 88-93

    8. Electrotransformation of Acidophilic, Heterotrophic, Gram-negative Bacteria

      • Thomas E. Ward
      Pages 94-103

    9. Acetobacter xylinum — Biotechnology and Food Technology

      • Robert E. Cannon
      Pages 104-107

    10. Electrotransformation of Sphingomonas paucimobilis

      • Isabel Sá-Correia, Arsénio M. Fialho
      Pages 108-118

    11. Bacillus amyloliquefaciens — Production Host for Industrial Enzymes

      • Jari Vehmaanperä
      Pages 119-123

  4. Medical and Veterinary Applications

    1. Front Matter

      Pages 125-125
      Download chapter PDF

    2. Electrotransformation of Yersinia ruckeri

      • Juan M. Cutrín, Alicia E. Toranzo, Juan L. Barja
      Pages 127-133

    3. Electrotransformation of Enterococci

      • Albrecht Muscholl-Silberhorn, Reinhard Wirth
      Pages 134-139
Back to top

Keywords

About this book

Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduce genetic material into bacterial cells. A strong electric field is used for this purpose.
In the present manual, protocols for the transformation of about 40 strains of bacteria are described. Emphasis is placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows the user to modify and adapt each protocol to his/her own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.

Editors and Affiliations

  • Institut de Pharmacologie et Biologie Structurale, CNRS UPR 9062, Toulouse cédex, France

    Natalie Eynard, Justin Teissié

Bibliographic Information

  • Book Title: Electrotransformation of Bacteria

  • Editors: Natalie Eynard, Justin Teissié

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-04305-9

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Softcover ISBN: 978-3-540-66680-6Published: 28 June 2000

  • eBook ISBN: 978-3-662-04305-9Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XI, 292

  • Number of Illustrations: 8 b/w illustrations

  • Topics: Biotechnology, Microbiology, Molecular Medicine, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation