Happy holidays from us to you—get up to $30 off your next print or eBook! Shop now >>

Springer Lab Manuals

Flow Cytometry and Cell Sorting

Editors: Radbruch, Andreas (Ed.)

Buy this book

eBook $259.00
price for USA in USD (gross)
  • ISBN 978-3-662-04129-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $329.99
price for USA in USD
  • ISBN 978-3-642-08492-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $349.99
price for USA in USD
  • ISBN 978-3-540-65630-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
From the reviews of the 1st edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended and well worth purchasing." (Cytobios, GB)
"Full practical details and useful hints are given. This book will prove to be very useful in the lab." (Comp Biochem Physiol A, GB)
"..the book provides a good overview of many of the uses of flow cytometry and would certainly be a good starting point for any of the topics covered. It is easy to read and as such would provide a good introduction to newcomers to flow cytometry." (Austr J Med Science)

Table of contents (26 chapters)

  • Operation of a Flow Cytometer

    Göttlinger, Christoph (et al.)

    Pages 3-25

  • Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies

    Müller, Werner

    Pages 29-37

  • Immunofluorescence: Basic Considerations

    Radbruch, Andreas

    Pages 38-52

  • Multicolor Immunofluorescence Analysis

    Chen, Chia-Huei (et al.)

    Pages 53-62

  • Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells

    Assenmacher, Mario

    Pages 63-70

Buy this book

eBook $259.00
price for USA in USD (gross)
  • ISBN 978-3-662-04129-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $329.99
price for USA in USD
  • ISBN 978-3-642-08492-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $349.99
price for USA in USD
  • ISBN 978-3-540-65630-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Flow Cytometry and Cell Sorting
Editors
  • Andreas Radbruch
Series Title
Springer Lab Manuals
Copyright
2000
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-04129-1
DOI
10.1007/978-3-662-04129-1
Softcover ISBN
978-3-642-08492-8
Softcover ISBN
978-3-540-65630-2
Edition Number
2
Number of Pages
XXIII, 355
Topics