PCR Topics

Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

Editors: Rolfs, Arndt, Schumacher, Hermann C., Marx, Peter (Eds.)

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About this book

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Table of contents (43 chapters)

Table of contents (43 chapters)
  • Quantitation of mRNA by the Polymerase Chain Reaction

    Pages 3-8

    Wang, Alice M. (et al.)

  • Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes

    Pages 9-14

    Buerstedde, Jean-Marie (et al.)

  • Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR)

    Pages 15-22

    Hampl, Hartmut (et al.)

  • Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene

    Pages 23-31

    Berg, Lutz-Peter (et al.)

  • The Use of PCR in the Diagnosis of Muscle Disease

    Pages 32-40

    Ehrenpreis, Jörg (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-3-642-75924-6
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.00
price for USA in USD
  • ISBN 978-3-540-52934-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
PCR Topics
Book Subtitle
Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases
Editors
  • Arndt Rolfs
  • Hermann C. Schumacher
  • Peter Marx
Copyright
1991
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-75924-6
DOI
10.1007/978-3-642-75924-6
Softcover ISBN
978-3-540-52934-7
Edition Number
1
Number of Pages
XI, 258
Number of Illustrations
50 b/w illustrations
Topics