Principles and Practice

Mammalian and Avian Transgenesis - New Approaches

Editors: Pease, Shirley, Lois, Carlos (Eds.)

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eBook $219.00
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  • ISBN 978-3-540-28489-5
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Hardcover $279.99
price for USA in USD
  • ISBN 978-3-540-28415-4
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Softcover $279.99
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  • ISBN 978-3-642-06665-8
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About this book

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.

The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Table of contents (11 chapters)

  • Generation of Transgenic Animals Using Lentiviral Vectors

    Lois, Carlos

    Pages 1-22

  • Intracytoplasmic Sperm Injection (ICSI) in the Mouse

    Li, Ming-Wen (et al.)

    Pages 23-40

  • Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation

    Eggan, Kevin (et al.)

    Pages 41-68

  • Cloning The Laboratory Mouse by Nuclear Transfer

    Eggan, Kevin (et al.)

    Pages 69-96

  • Large Insert Transgenesis

    Gong, Shiaoching (et al.)

    Pages 97-110

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-3-540-28489-5
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-3-540-28415-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $279.99
price for USA in USD
  • ISBN 978-3-642-06665-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mammalian and Avian Transgenesis - New Approaches
Editors
  • Shirley Pease
  • Carlos Lois
Series Title
Principles and Practice
Copyright
2006
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-540-28489-5
DOI
10.1007/978-3-540-28489-5
Hardcover ISBN
978-3-540-28415-4
Softcover ISBN
978-3-642-06665-8
Series ISSN
1866-914X
Edition Number
1
Number of Pages
XX, 281
Number of Illustrations
36 b/w illustrations, 12 illustrations in colour
Topics