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Diffusion Chamber Culture

Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays

Editors: Cronkite, E. P., Carsten, A. L. (Eds.)

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About this book

Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a result of our deep interest in and involvement with diffusion chamber culture Dr. A. L. Carsten and I organized this conference. A major objective of this conference was to seek answers to the above question.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • History and Overview of the In Vivo Diffusion Chamber (DC) Culture System

    Pages 3-10

    Carsten, A. L.

  • Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrow

    Pages 11-18

    Shulman, L. N. (et al.)

  • Different Classes of Granulocyte Progenitor Cells Expressed in In Vivo Plasma Clot Diffusion Chamber Cultures

    Pages 19-27

    Steinberg, H. N. (et al.)

  • Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambers

    Pages 28-43

    Jacobsen, N.

  • Studies on the Regulation of Diffusion Chamber Granulopoiesis

    Pages 44-56

    Quesenberry, P. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-3-642-67644-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.00
price for USA in USD
  • ISBN 978-3-540-10064-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Diffusion Chamber Culture
Book Subtitle
Hemopoiesis, Cloning of Tumors, Cytogenetic and Carcinogenic Assays
Editors
  • E. P. Cronkite
  • A. L. Carsten
Copyright
1980
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-642-67644-4
DOI
10.1007/978-3-642-67644-4
Softcover ISBN
978-3-540-10064-5
Edition Number
1
Number of Pages
XIV, 277
Number of Illustrations
12 b/w illustrations
Topics

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