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Techniques in Life Science and Biomedicine for the Non-Expert

Flow Cytometry Basics for the Non-Expert

Authors: Goetz, Christine, Hammerbeck, Christopher, Bonnevier, Jody

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  • Includes tips on how to set up an experiment for flow cytometry optimially
  • Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens)
  • Section devoted to antibody development and conjugation
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eBook  
  • ISBN 978-3-319-98071-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover $89.99
price for USA in USD
  • ISBN 978-3-319-98070-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly.

The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays.  Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques.

Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data.  This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.  

About the authors

Christine Goetz, Ph.D., Jody Bonnevier, Ph.D., and Christopher Hammerbeck, Ph.D.

R&D Systems, Minneapolis, Minnesota



Table of contents (10 chapters)

Table of contents (10 chapters)
  • Flow Cytometry: Definition, History, and Uses in Biological Research

    Pages 1-11

    Bonnevier, Jody (et al.)

  • Physics of a Flow Cytometer

    Pages 13-24

    Bonnevier, Jody (et al.)

  • The Language of Flow Cytometry and Experimental Setup

    Pages 25-40

    Goetz, Christine (et al.)

  • Fluorochrome Choices for Flow Cytometry

    Pages 41-51

    Goetz, Christine (et al.)

  • Using the Process of Compensation to Prevent False Positive Data Caused by Fluorescence Spillover: A Practical Example

    Pages 53-74

    Hammerbeck, Christopher (et al.)

Buy this book

eBook  
  • ISBN 978-3-319-98071-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover $89.99
price for USA in USD
  • ISBN 978-3-319-98070-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Flow Cytometry Basics for the Non-Expert
Authors
Series Title
Techniques in Life Science and Biomedicine for the Non-Expert
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-319-98071-3
DOI
10.1007/978-3-319-98071-3
Hardcover ISBN
978-3-319-98070-6
Series ISSN
2367-1114
Edition Number
1
Number of Pages
XVII, 219
Number of Illustrations
4 b/w illustrations, 113 illustrations in colour
Topics