Springer Theses

Imaging, Manipulation and Optogenetics in Zebrafish

Authors: Favre‐Bulle, Itia Amandine

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  • Nominated as an outstanding Ph.D thesis by the University of Queensland, Sydney, Australia
  • Develops and combines novel optical methods for micromanipulation of neuronal system 
  • Describes an intensively pursued research area that promises important advances in basic neuroscience
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  • ISBN 978-3-319-96250-4
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About this book

The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

Table of contents (7 chapters)

Table of contents (7 chapters)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-3-319-96250-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-3-319-96249-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Imaging, Manipulation and Optogenetics in Zebrafish
Authors
Series Title
Springer Theses
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-319-96250-4
DOI
10.1007/978-3-319-96250-4
Hardcover ISBN
978-3-319-96249-8
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XVIII, 86
Number of Illustrations
26 b/w illustrations, 23 illustrations in colour
Topics