Read While You Wait - Get immediate ebook access, if available*, when you order a print book

Springer Theses

Fluorescent Tools for Imaging Oxidative Stress in Biology

Authors: Kaur, Amandeep

Free Preview
  • Nominated as an outstanding Ph.D. thesis by the University of Sydney, Australia
  • Outlines new reversible fluorescent redox sensors to visualise subcellular oxidative stress
  • Demonstrates successful applications of redox sensors in a variety of biological systems
  • Provides clear explanations for chemists and biologists alike
see more benefits

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-3-319-73405-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-3-319-73404-0
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-3-030-10371-2
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
About this book

This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases.

The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies.

In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research.

About the authors

Amandeep Kaur completed her master’s studies in chemistry, with a University medal from VIT University, India in 2012. She then took up a research internship at ENSCCF, France, where she worked on the development of transition metal-doped calcium phosphate ceramics. Amandeep completed her Ph.D. in 2016 at the University of Sydney with Dr Elizabeth New, during which she was awarded a World Scholars Scholarship. Her Ph.D. research involved the development of reversible fluorescent redox sensors for understanding the role of oxidative stress in biological systems. She is currently working as a postdoctoral research fellow at the EMBL Australia Node in Single Molecule Science, University of New South Wales, Australia.

Table of contents (10 chapters)

Table of contents (10 chapters)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-3-319-73405-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-3-319-73404-0
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-3-030-10371-2
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Fluorescent Tools for Imaging Oxidative Stress in Biology
Authors
Series Title
Springer Theses
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing AG
eBook ISBN
978-3-319-73405-7
DOI
10.1007/978-3-319-73405-7
Hardcover ISBN
978-3-319-73404-0
Softcover ISBN
978-3-030-10371-2
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XLV, 230
Number of Illustrations
61 b/w illustrations, 100 illustrations in colour
Topics

*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works and instructor copies are not included.