Biological and Medical Physics, Biomedical Engineering

Membrane Proteins in Aqueous Solutions

From Detergents to Amphipols

Authors: Popot, Jean-Luc

  • First book dedicated to the art of handling membrane proteins in aqueous solutions 
  • Includes a Foreword by Donald M. Engelman and over 30 cartoons created by Francis Haraux
  • Includes over 300 richly illustrated figures and topical tables as well as twelve detailed, experimental protocols
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Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications.

Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users.

Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.


About the authors

Jean-Luc Popot, born 1948, studied biology, chemistry, and biophysics in Orléans and Orsay. In 1971, he took a permanent position with the Collège de France. In J.‑P. Changeux’s laboratory, at the Pasteur Institute, he applied electrophysiology and biophysical and biochemical approaches to functional and structural studies of the nicotinic acetyl­cho­li­ne receptor. In 1982, he joined D.M. Engelman at Yale University as a visiting scientist. His work at Yale, which bore princi­pal­ly on refolding bacterio­rhod­opsin from dena­tu­red fragments and studying the refolded structure crystallographically, led the two of them to propose, in 1990, an influential model for the folding of α‑helical membrane proteins. In 1985, he joined the laboratory of P. Joliot at the Institut de Biologie Physico-Chimique (Paris). His group car­ried out neutron diffraction and model building work on bacterio­rhod­opsin and bio­che­mical studies on Photo­sys­tem II and cytochrome bf. In 1996, he became Research Director at the Centre National de la Recher­­che Scienti­fi­que and created his own laboratory, where the X‑ray struc­tu­re of the bf was solved in 2003. In parallel, he pursued the development of sequence analysis approa­ches and, in collabo­ra­tion with chemists and physical chemists, designed and validated non-conventional surfact­ants aimed at facilitating membrane protein solu­tion studies, most notably amphi­­pathic polymers (‘amphipols’) and fluorinated surfactants. He retired in 2013 and, along with his wife, splits his time between restoring an old house in Languedoc, reading, writing, hiking, photographing, cooking, and enjoying the company of kith and kin.

Table of contents (15 chapters)

  • Membrane Proteins and Their Natural Environment

    Popot, Jean-Luc

    Pages 1-57

  • Extracting Membrane Proteins from Their Native Environment

    Popot, Jean-Luc

    Pages 59-95

  • Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions

    Popot, Jean-Luc

    Pages 97-149

  • Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols

    Popot, Jean-Luc

    Pages 151-236

  • Formation and Properties of Membrane Protein/Amphipol Complexes

    Popot, Jean-Luc

    Pages 237-332

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Proteins in Aqueous Solutions
Book Subtitle
From Detergents to Amphipols
Authors
Series Title
Biological and Medical Physics, Biomedical Engineering
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing AG, part of Springer Nature
eBook ISBN
978-3-319-73148-3
DOI
10.1007/978-3-319-73148-3
Hardcover ISBN
978-3-319-73146-9
Series ISSN
1618-7210
Edition Number
1
Number of Pages
XXVII, 708
Number of Illustrations and Tables
171 b/w illustrations, 266 illustrations in colour
Topics