Biological and Medical Physics, Biomedical Engineering

Membrane Proteins in Aqueous Solutions

From Detergents to Amphipols

Authors: Popot, Jean-Luc

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  • First book dedicated to the art of handling membrane proteins in aqueous solutions 
  • Includes a Foreword by Donald M. Engelman and over 30 cartoons created by Francis Haraux
  • Includes over 400 richly illustrated figures and topical tables as well as twelve detailed, experimental protocols
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book is the first to be entirely devoted to the challenging art of handling membrane proteins out of their natural environment, a key process in biological and pharmaceutical research, but one plagued with difficulties and pitfalls. Written by one of the foremost experts in the field, Membrane Proteins in Aqueous Solutions is accessible to any member of a membrane biology laboratory. After presenting the structure, functions, dynamics, synthesis, natural environment and lipid interactions of membrane proteins, the author discusses the principles of extracting them with detergents, the mechanisms of detergent-induced destabilization, countermeasures, and recent progress in developing detergents with weaker denaturing properties. Non-conventional alternatives to detergents, including bicelles, nanodiscs, amphipathic peptides, fluorinated surfactants and amphipols, are described, and their relative advantages and drawbacks are compared. The synthesis and solution properties of the various types of amphipols are presented, as well as the formation and properties of membrane protein/amphipol complexes and the transfer of amphipol-trapped proteins to detergents, nanodiscs, lipidic mesophases, or living cells. The final chapters of the book deal with applications: membrane protein in vitro folding and cell-free expression, solution studies, NMR, crystallography, electron microscopy, mass spectrometry, amphipol-mediated immobilization of membrane proteins, and biomedical applications.

Important features of the book include introductory sections describing foundations as well as the state-of-the-art for each of the biophysical techniques discussed, and topical tables which organize a widely dispersed literature. Boxes and annexes throughout the book explain technical aspects, and twelve detailed experimental protocols, ranging from in vitro folding of membrane proteins to single-particle electron cryomicroscopy, have been contributed by and commented on by experienced users.

Membrane Proteins in Aqueous Solutions offers a concise, accessible introduction to membrane protein biochemistry and biophysics, as well as comprehensive coverage of the properties and uses of conventional and non-conventional surfactants. It will be useful both in basic and applied research laboratories and as a teaching aid for students, instructors, researchers, and professionals within the field.


About the authors

Jean-Luc Popot, born 1948, studied biology, chemistry, and biophysics in Orléans and Orsay. In 1971, he took a permanent position with the Collège de France. In J.‑P. Changeux’s laboratory, at the Pasteur Institute, he applied electrophysiology and biophysical and biochemical approaches to functional and structural studies of the nicotinic acetyl­cho­li­ne receptor. In 1982, he joined D.M. Engelman at Yale University as a visiting scientist. His work at Yale, which bore princi­pal­ly on refolding bacterio­rhod­opsin from dena­tu­red fragments and studying the refolded structure crystallographically, led the two of them to propose, in 1990, an influential model for the folding of α‑helical membrane proteins. In 1985, he joined the laboratory of P. Joliot at the Institut de Biologie Physico-Chimique (Paris). His group car­ried out neutron diffraction and model building work on bacterio­rhod­opsin and bio­che­mical studies on Photo­sys­tem II and cytochrome bf. In 1996, he became Research Director at the Centre National de la Recher­­che Scienti­fi­que and created his own laboratory, where the X‑ray struc­tu­re of the bf was solved in 2003. In parallel, he pursued the development of sequence analysis approa­ches and, in collabo­ra­tion with chemists and physical chemists, designed and validated non-conventional surfact­ants aimed at facilitating membrane protein solu­tion studies, most notably amphi­­pathic polymers (‘amphipols’) and fluorinated surfactants. He retired in 2013 and, along with his wife, splits his time between restoring an old house in Languedoc, reading, writing, hiking, photographing, cooking, and enjoying the company of kith and kin.

Reviews

“Jean-Luc Popot writes with the natural authority that comes from a long career as a pioneer in membrane protein biophysics. His prose has the exact and wry elegance of a true scholar and a flow, stringency and clarity that people will recognize from his conference lectures. He is a true exponent of the classical French academic tradition for establishing fundamental underlying principles. The wonderful and subtly informative cartoons by Francis Haraux, which complement the judiciously chosen scientific figures, make a perfect companion to this unfolding story. The main topic of the book is the use of amphipols to advance our understanding of membrane proteins; this remarkable group of molecules is a true brain child of Dr. Popot and it is in large part his efforts which are responsible for their introduction into the mainstay of membrane protein science. This is undoubtedly the best overview of their properties and it is a true privilege for the reader to have access to a monograph (an increasingly rare phenomenon) with such unity of purpose and direction. Many instructive hours of reading are guaranteed!” (Daniel E. Otzen, Aarhus University, Aarhus, Denmark)

“This book is fully written by a single author, Jean-Luc Popot, a pioneer in the field of membrane protein biophysics who contributed seminal work that illuminates how membrane proteins fold and who spent a career exploring how they can best be handled.  In the latter area, his signature accomplishment was to lead the development and painstaking characterization of amphipathic polymers—amphipols—for use as a membrane mimetic medium.  While this work presents a definitive account of the properties and applications of amphipols, it also includes several introductory chapters that lay out both the science and history of working with membrane proteins. It represents a formidable work of scholarship, with all chapters being accompanied by an engaging series of cartoons by Francis Haraux.  At the same time, it is packed with useful practical information on membrane proteins and model membranes.   A classic work.” (Charles R. Sanders, Vanderbilt School of Medicine, Nashville, USA)

“This most valuable book covers biophysical chemistry of membrane proteins,from progress in conceptual understanding of molecular structure and mechanisms to comprehensive overview of traditional and novel experimental methods. Must-have for every laboratory interested in membrane proteins.” (Ilia Denisov, The University of Illinois, Urbana-Champaign, USA)

“The book on membrane proteins by Popot provides a wonderful, comprehensive overview alongside very detailed insights into the behaviour and properties of membrane proteins once they have been extracted from their normal membrane environment.  It is a "must-read" for all practical experimentalists working on membrane protein structure and function.” (Richard Henderson, University of Cambridge, UK)

“This important work by Popot provides a timely and comprehensive communication regarding membrane proteins, which constitute a majority of targets for therapeutic intervention.  Well referenced and with an unusual ability to communicate complex phenomena in a clear and concise manner, this book should be on the shelf of all biochemists, pharmacologists, chemists and biologists.” (Stephen Sligar, The University of Illinois, Urbana-Champaign, USA)

“The foundation of successful membrane protein biochemistry and structural biology is an in-depth understanding of detergents and surfactants. Chapter 2 gives an essential introduction into their biophysical characteristics and biochemical uses. The chapter gives the rationale to crucial observations in membrane protein purification, such as why adding too much or too little detergent can be a very bad thing and why using the right detergent at the correct concentration is critical for purification of membrane proteins in a functional state. I thoroughly recommend this chapter as essential reading for anyone embarking into the challenging world of membrane protein purification.” (Christopher G. Tate, University of Cambridge, UK)

Table of contents (15 chapters)

Table of contents (15 chapters)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-3-319-73148-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-3-319-73146-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Proteins in Aqueous Solutions
Book Subtitle
From Detergents to Amphipols
Authors
Series Title
Biological and Medical Physics, Biomedical Engineering
Copyright
2018
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing AG, part of Springer Nature
eBook ISBN
978-3-319-73148-3
DOI
10.1007/978-3-319-73148-3
Hardcover ISBN
978-3-319-73146-9
Series ISSN
1618-7210
Edition Number
1
Number of Pages
XXVII, 708
Number of Illustrations
171 b/w illustrations, 266 illustrations in colour
Topics