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Rolling Circle Amplification (RCA)

Toward New Clinical Diagnostics and Therapeutics

Editors: Demidov, Vadim V. (Ed.)

  • Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells, along with DNA nanoclews and DNA origami nanostructrures as vehicles for intracellular delivery of various drugs
  • Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads
  • Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells, ramified RCA for identifying the disease-related SNPs, RNA-primed RCA for detection of microbial pathogens and in situ RCA diagnosis of cancer cells and malignant tissues
  • Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification
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Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-3-319-42226-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-3-319-42224-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-3-319-82529-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensors for expediting RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies.
Specifically, this book:

  • Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells
  • Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads
  • Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells and in situ RCA diagnosis of cancer cells and malignant tissues
  • Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification

Advance Praise for Rolling Circle Amplification (RCA):
“This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs.”— Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA)
“In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts for building superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology.”— Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.

About the authors

Vadim V. Demidov, Ph.D., is a Senior Analyst in the Biotechnology & Pharmaceuticals Group at Global Prior Art, Inc. (Boston, USA), an intellectual property research and analysis firm. Dr. Demidov received his M.S. degree in Physical and Chemical Engineering from Moscow Institute of Physics and Technology (MIPT, a leading Russian technical university, also known as “the Russian MIT”) and his Ph.D. degree in Biophysics from the Institute of Molecular Genetics of the Russian Academy of Sciences (IMGRAS) and MIPT.
Before joining the Global Prior Art company in 2008, he worked for almost 30 years in academia and industry worldwide. Dr. Demidov is well known in the molecular biology and biotechnology field for his innovative studies related to peptide nucleic acid (PNA). He has introduced the use of PNA openers for detection of specific sequences within DNA duplexes under non-denaturing conditions, with some of these applications involving rolling circle amplification (RCA).

Table of contents (14 chapters)

  • Introduction: 20+ Years of Rolling the DNA Minicircles—State of the Art in the RCA-Based Nucleic Acid Diagnostics and Therapeutics

    Demidov, Vadim V.

    Pages 1-7

  • Improvement of ϕ29 DNA Polymerase Amplification Performance by Fusion of DNA Binding Motifs

    Vega, Miguel, Ph.D. in Biology (et al.)

    Pages 11-24

  • Preparation of Circular Templates by T4 RNA Ligase 2 for Rolling Circle Amplification of Target microRNAs with High Specificity and Sensitivity

    Guan, Yifu, Ph.D. (et al.)

    Pages 25-35

  • Use of DNA CircLigase for Direct Isothermal Detection of Microbial mRNAs by RNA-Primed Rolling Circle Amplification and Preparation of ø29 DNA Polymerase Not Contaminated by Amplifiable DNA

    Takahashi, Hirokazu, Ph.D. (et al.)

    Pages 37-46

  • Nicking Enzyme-Assisted Branched-Chain RCA Reaction for Cascade DNA Amplification

    Zhu, Xiaoli (et al.)

    Pages 49-56

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-3-319-42226-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-3-319-42224-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-3-319-82529-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rolling Circle Amplification (RCA)
Book Subtitle
Toward New Clinical Diagnostics and Therapeutics
Editors
  • Vadim V. Demidov
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-42226-8
DOI
10.1007/978-3-319-42226-8
Hardcover ISBN
978-3-319-42224-4
Softcover ISBN
978-3-319-82529-8
Edition Number
1
Number of Pages
XVII, 176
Number of Illustrations
16 b/w illustrations, 37 illustrations in colour
Topics