Overview
- Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells, along with DNA nanoclews and DNA origami nanostructrures as vehicles for intracellular delivery of various drugs
- Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads
- Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells, ramified RCA for identifying the disease-related SNPs, RNA-primed RCA for detection of microbial pathogens and in situ RCA diagnosis of cancer cells and malignant tissues
- Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification
- Includes supplementary material: sn.pub/extras
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Table of contents (14 chapters)
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Front Matter
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Improved DNA Polymerases and New DNA and DNA/RNA Ligases Useful in RCA
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Front Matter
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RCA-Involving Techniques with Enhanced Signal Amplification
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Front Matter
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Emerging RCA Diagnostics
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Front Matter
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Prospective RCA-Based Therapeutics
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Front Matter
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Back Matter
Keywords
- Coupled rolling circle amplification
- Enzymes Useful in RCA
- Improved variants of φ29 polymerase
- Intracellular Delivery of Immunostimulatory Drugs
- Novel RCA diagnostics
- PNA Openers-Assisted RCA
- Prospective RCA-based therapeutics
- RCA
- RCA-Based DNA Origami Nanostructrures
- RCA-LAMP
- RCA-Templated DNA Nanotubes
- RCA-based analysis of hepatitis B virus (HBV)
- RCA-directed enzyme-catalyzed polymerization
- Rolling circle amplification
- Sensors and detector probes for RCA detection
- Thermus thermophilus SSB mutant protein
- assays in nucleic acid diagnostics
- probe-tagged magnetic nanobeads
- prospective RCA-based therapeutics
- real-time monitoring of rolling-circle DNA amplification
About this book
This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensors for expediting RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies.
Specifically, this book:
Advance Praise for Rolling Circle Amplification (RCA):
“This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs.”
— Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA)
“In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts forbuilding superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology.”
— Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.
Specifically, this book:
- Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells
- Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads
- Maximizes reader insights into novel RCA diagnostics,such as PNA openers-assisted RCA for detection of single target cells and in situ RCA diagnosis of cancer cells and malignant tissues
- Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification
Advance Praise for Rolling Circle Amplification (RCA):
“This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs.”
— Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA)
“In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts forbuilding superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology.”
— Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.
Editors and Affiliations
About the editor
Vadim V. Demidov, Ph.D., is a Senior Analyst in the Biotechnology & Pharmaceuticals Group at Global Prior Art, Inc. (Boston, USA), an intellectual property research and analysis firm. Dr. Demidov received his M.S. degree in Physical and Chemical Engineering from Moscow Institute of Physics and Technology (MIPT, a leading Russian technical university, also known as “the Russian MIT”) and his Ph.D. degree in Biophysics from the Institute of Molecular Genetics of the Russian Academy of Sciences (IMGRAS) and MIPT.
Before joining the Global Prior Art company in 2008, he worked for almost 30 years in academia and industry worldwide. Dr. Demidov is well known in the molecular biology and biotechnology field for his innovative studies related to peptide nucleic acid (PNA). He has introduced the use of PNA openers for detection of specific sequences within DNA duplexes under non-denaturing conditions, with some of these applications involving rolling circle amplification (RCA).
Before joining the Global Prior Art company in 2008, he worked for almost 30 years in academia and industry worldwide. Dr. Demidov is well known in the molecular biology and biotechnology field for his innovative studies related to peptide nucleic acid (PNA). He has introduced the use of PNA openers for detection of specific sequences within DNA duplexes under non-denaturing conditions, with some of these applications involving rolling circle amplification (RCA).
Bibliographic Information
Book Title: Rolling Circle Amplification (RCA)
Book Subtitle: Toward New Clinical Diagnostics and Therapeutics
Editors: Vadim V. Demidov
DOI: https://doi.org/10.1007/978-3-319-42226-8
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer International Publishing Switzerland 2016
Hardcover ISBN: 978-3-319-42224-4Published: 17 November 2016
Softcover ISBN: 978-3-319-82529-8Published: 07 July 2018
eBook ISBN: 978-3-319-42226-8Published: 09 November 2016
Edition Number: 1
Number of Pages: XVII, 176
Number of Illustrations: 16 b/w illustrations, 37 illustrations in colour
Topics: Enzymology, Biochemical Engineering, Medical Biochemistry