Springer Theses

Nanoscale Imaging and Characterisation of Amyloid-β

Authors: Tinker-Mill, Claire Louisa

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  • Nominated as an outstanding Ph.D. thesis by the Lancaster University, UK
  • Presents a detailed study of amyloid-beta using multiple methods of atomic force microscopy
  • Author received the 2012 and 2014 Juno Award for Research Excellence from Lancaster University
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-3-319-39534-0
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Hardcover $139.99
price for USA in USD
  • ISBN 978-3-319-39533-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.99
price for USA in USD
  • ISBN 978-3-319-81907-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide’s nanomechanical, elastic, thermal or spectroscopical properties. 
Amyloid-β (Aβ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Aβ with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.

Table of contents (9 chapters)

Table of contents (9 chapters)
  • Introduction

    Tinker-Mill, Claire Louisa

    Pages 1-5

  • Theoretical Concepts of Scanning Probe Microscopy and Dynamic Light Scattering and Their Relation to the Study of Peptide Nanostructures

    Tinker-Mill, Claire Louisa

    Pages 7-30

  • Alzheimer’s Disease and the Aggregation of Amyloid β

    Tinker-Mill, Claire Louisa

    Pages 31-52

  • Experimental Methodology

    Tinker-Mill, Claire Louisa

    Pages 53-72

  • Substrate Development of the Imaging of Amyloid Proteins with SPM Methods

    Tinker-Mill, Claire Louisa

    Pages 73-86

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-3-319-39534-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-3-319-39533-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.99
price for USA in USD
  • ISBN 978-3-319-81907-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nanoscale Imaging and Characterisation of Amyloid-β
Authors
Series Title
Springer Theses
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-39534-0
DOI
10.1007/978-3-319-39534-0
Hardcover ISBN
978-3-319-39533-3
Softcover ISBN
978-3-319-81907-5
Series ISSN
2190-5053
Edition Number
1
Number of Pages
XX, 149
Number of Illustrations
45 b/w illustrations, 14 illustrations in colour
Topics