Advances in Experimental Medicine and Biology

The Next Generation in Membrane Protein Structure Determination

Editors: Moraes, Isabel (Ed.)

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  • Gives novel approaches to structure determination of membrane proteinsProvides good coverage on how membrane proteins interact with their surrounding environment
  • Is a useful guide not only for students, but also for experienced scientists who need to deal with very small and problematic protein crystals
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eBook $139.00
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  • ISBN 978-3-319-35072-1
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Hardcover $179.99
price for USA in USD
  • ISBN 978-3-319-35070-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
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  • ISBN 978-3-319-81715-6
  • Free shipping for individuals worldwide
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About this book

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects.  Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins.

This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes:

  • Expression screening using fluorescent proteins
  • The use of detergents in membrane protein research
  • The use of NMR
  • Synchrotron developments in membrane protein structural biology
  • Visualisation and X-ray data collection of microcrystals
  • X-ray diffraction data analysis from multiple crystals
  • Serial millisecond crystallography
  • Serial femtosecond crystallography
  • Membrane protein structures in drug discovery

The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work.

Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.


Table of contents (14 chapters)

Table of contents (14 chapters)
  • Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters

    Bird, Louise E. (et al.)

    Pages 1-11

  • Detergents in Membrane Protein Purification and Crystallisation

    Anandan, Anandhi (et al.)

    Pages 13-28

  • NMR of Membrane Proteins: Beyond Crystals

    Rajesh, Sundaresan (et al.)

    Pages 29-42

  • Characterisation of Conformational and Ligand Binding Properties of Membrane Proteins Using Synchrotron Radiation Circular Dichroism (SRCD)

    Hussain, Rohanah (et al.)

    Pages 43-59

  • Membrane Protein Crystallisation: Current Trends and Future Perspectives

    Parker, Joanne L. (et al.)

    Pages 61-72

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-3-319-35072-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-3-319-35070-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-3-319-81715-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Next Generation in Membrane Protein Structure Determination
Editors
  • Isabel Moraes
Series Title
Advances in Experimental Medicine and Biology
Series Volume
922
Copyright
2016
Publisher
Springer International Publishing
Copyright Holder
Springer International Publishing Switzerland
eBook ISBN
978-3-319-35072-1
DOI
10.1007/978-3-319-35072-1
Hardcover ISBN
978-3-319-35070-7
Softcover ISBN
978-3-319-81715-6
Series ISSN
0065-2598
Edition Number
1
Number of Pages
X, 184
Number of Illustrations
73 b/w illustrations
Topics