Pre-Analytics of Pathological Specimens in Oncology

1. Front Matter

   Pages i-vi

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2. The Pre-analytical Phase in Surgical Pathology

   • Gianni Bussolati, Laura Annaratone, Francesca Maletta

   Pages 1-13

3. Histologic Validation of Vacuum Sealed, Formalin-Free Tissue Preservation, and Transport System

   • Richard J. Zarbo

   Pages 15-26

4. The Evolution of Pre-analytic Factors in Anatomic Pathology

   • Stephen M. Hewitt

   Pages 27-34

5. Tissue Heterogeneity as a Pre-analytical Source of Variability

   • Giorgio Stanta

   Pages 35-43

6. Standardized Processing of Native Tissue in Breast Pathology

   • M. E. Dämmrich, H. H. Kreipe

   Pages 45-53

7. Short Formalin Fixation and Rapid Microwave Processing Do Not Affect HER2 Testing

   • Isabella Sassi, Francesca Invernizzi, Claudio Doglioni

   Pages 55-64

8. Current Projects in Pre-analytics: Where to Go?

   • Anna Sapino, Laura Annaratone, Caterina Marchiò

   Pages 65-70

9. Preanalytics in Lung Cancer

   • Arne Warth, Thomas Muley, Michael Meister, Wilko Weichert

   Pages 71-84

10. RNA Quality in Fresh-Frozen Gastrointestinal Tumor Specimens—Experiences from the Tumor and Healthy Tissue Bank TU Dresden

    • Silke Zeugner, Thomas Mayr, Christian Zietz, Daniela E. Aust, Gustavo B. Baretton

    Pages 85-93

11. Bone Marrow Work-up: Report of a Pilot Study

    • Ioannis Anagnostopoulos, Dido Lenze, Michael Hummel, Manfred Dietel, Korinna Jöhrens

    Pages 95-105

12. Two-Temperature Formalin Fixation Preserves Activation States Efficiently

    • David Chafin

    Pages 107-117

13. Towards the Lean lab: The Industry Challenge

    • Franco Visinoni

    Pages 119-133

This book presents an overview of the most important current developments in the pre-analytical handling of tissue. It addresses in particular potential ways to improve the situation whereby methods employed in the pre-analytical phase – the period from surgical removal of tissue to the start of pathological processing – have remained essentially unchanged for decades with only modest standardization. It is examined how the pre-analytical period can be optimized, resulting not only in an increase in diagnostic quality but also in a reduction in processing time and costs. Among the key topics examined are the so-called cold ischemia time between tissue removal and fixation, the potential superiority of vacuum-based preservation over immediate formalin fixation, two-temperature fixation, molecular analysis methods, and the pre-analytics of specimens from particular tissues. Readers will find this book to be an important update that reveals the full importance of the pre-analytical phase for quality of pathological work-up.

• Cold Ischemia Time
• Molecular Analysis Methods
• Preservation of Tissue
• Two-Temperature Fixation
• Vacuum-based Preservation

• Institute of Pathology, Charité University Medicine, Berlin, Germany

  Manfred Dietel, Moritz Winterfeld

• Institute of Pathology, University Hospital Leipzig, Leipzig, Germany

  Christian Wittekind

• University of Turin Institute of Pathology, Turin, Italy

  Gianni Bussolati

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