Overview
- Editors:
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Hans Jörnvall
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Department of Chemistry I, Karolinska Institute, Stockholm, Sweden
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Jan-Olov Höög
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Department of Chemistry I, Karolinska Institute, Stockholm, Sweden
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Ann-Margreth Gustavsson
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Department of Chemistry I, Karolinska Institute, Stockholm, Sweden
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Table of contents (39 chapters)
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Sequencer Methodology and Instrumentation
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- Brigitte Wittmann-Liebold, Lothar Matschull, Ulrike Pilling, Hans-Arthur Bradaczek, Horst Graffunder
Pages 9-21
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- Adam S. Inglis, Robert L. Moritz, Geoffrey S. Begg, Gavin E. Reid, Richard J. Simpson, Horst Graffunder et al.
Pages 23-34
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- David H. Hawke, Victoria L. Boyd
Pages 35-45
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- Richard A. Laursen, Thomas T. Lee, James D. Dixon, Song-Ping Liang
Pages 47-54
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- H. Reinke, St. Fischer, F. Reimann, H. Tschesche
Pages 55-66
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Sample Preparation and Analysis
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- Richard J. Simpson, Robert L. Moritz, Gavin E. Reid, Larry D. Ward
Pages 67-77
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- H. H. Rasmussen, J. Van Damme, G. Bauw, M. Puype, B. Gesser, J. E. Celis et al.
Pages 103-114
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- Ronald L. Niece, Lowell H. Ericsson, Audree V. Fowler, Alan J. Smith, David W. Speicher, John W. Crabb et al.
Pages 133-141
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- L. H. Ericsson, D. Atherton, R. Kutny, A. J. Smith, J. W. Crabb
Pages 143-150
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Modified Residues, Chemical Problems and Synthetic Peptides
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- H. Weiner, S. C. Cunningham, R. A. Angelo
Pages 161-167
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- H. Ponstingl, L. D. Barnes, C. Granzow, R. H. Himes, G. Maier, G. Nasioulas
Pages 169-176
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- E. Appella, J. G. Omichinski, G. M. Clore, A. M. Gronenborn, K. Sakaguchi
Pages 187-195
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- Jan Johansson, Tore Curstedt, Per Persson, Bengt Robertson, Björn Löwenadler, Hans Jörnvall
Pages 197-204
About this book
Methods in protein sequence analysis constitute important fields in rapid progress. We have experienced a continuous increase in analytical sensitivity coupled with decreases in time necessary for purification and analysis. Several generations of sequencers, liquid/solid/gas-phase, have passed by and returned in other shapes during just over two decades. Similarly, the introduction of HPLC permitted an enormous leap forward in this as in other fields of biochemistry, and we now start to see new major advances in purification/analysis through capillary electrophoresis. Furthermore, progress in the field of mass spectrometry has matched that in chemical analysis and we witness continuous development, now emphasizing ion spray and other mass spectrometric approaches. In short, protein analysis has progressed in line with other developments in modern science and constitutes an indispensable, integral part of present-day molecular biology. Even the available molecular tools, in the form of proteases with different specificities, have increased in number, although we still have far to go to reach an array of "restriction proteases" like the sets of nucleases available to the molecular geneticist. Of course, conferences have been devoted to protein sequence analysis, in particular the MPSA (Methods in Protein Sequence Analysis) series, of which the 8th conference took place in Kiruna, Sweden, July 1-6 1990. Again, we witnessed much progress, saw new instruments, and experienced further interpretational insights into protein mechanisms and functions.