Advances in Life Sciences

Methods in Protein Sequence Analysis

Authors: Jörnvall, Höög

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About this book

Methods in protein sequence analysis constitute important fields in rapid progress. We have experienced a continuous increase in analytical sensitivity coupled with decreases in time necessary for purification and analysis. Several generations of sequencers, liquid/solid/gas-phase, have passed by and returned in other shapes during just over two decades. Similarly, the introduction of HPLC permitted an enormous leap forward in this as in other fields of biochemistry, and we now start to see new major advances in purification/analysis through capillary electrophoresis. Furthermore, progress in the field of mass spectrometry has matched that in chemical analysis and we witness continuous development, now emphasizing ion spray and other mass spectrometric approaches. In short, protein analysis has progressed in line with other developments in modern science and constitutes an indispensable, integral part of present-day molecular biology. Even the available molecular tools, in the form of proteases with different specificities, have increased in number, although we still have far to go to reach an array of "restriction proteases" like the sets of nucleases available to the molecular geneticist. Of course, conferences have been devoted to protein sequence analysis, in particular the MPSA (Methods in Protein Sequence Analysis) series, of which the 8th conference took place in Kiruna, Sweden, July 1-6 1990. Again, we witnessed much progress, saw new instruments, and experienced further interpretational insights into protein mechanisms and functions.

Table of contents (39 chapters)

Table of contents (39 chapters)
  • Modular Berlin Microsequencer for the Sequential Degradation of Proteins and Peptides from the Amino- and Carboxyl-Terminal End

    Pages 9-21

    Wittmann-Liebold, Brigitte (et al.)

  • C-Terminal Sequence Analysis

    Pages 23-34

    Inglis, Adam S. (et al.)

  • Chemical C-Terminal Sequencing

    Pages 35-45

    Hawke, David H. (et al.)

  • Extending the Performance of the Solid-Phase Protein Sequencer

    Pages 47-54

    Laursen, Richard A. (et al.)

  • Direct Microsequencing of Blotted and Covalently Attached Proteins in a Cross-Flow Reaction Chamber

    Pages 55-66

    Reinke, H. (et al.)

Buy this book

eBook $79.99
price for USA in USD
  • ISBN 978-3-0348-5678-2
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $99.99
price for USA in USD
  • ISBN 978-3-0348-5680-5
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Methods in Protein Sequence Analysis
Authors
Series Title
Advances in Life Sciences
Copyright
1991
Publisher
Birkhäuser Basel
Copyright Holder
Springer Basel AG
eBook ISBN
978-3-0348-5678-2
DOI
10.1007/978-3-0348-5678-2
Softcover ISBN
978-3-0348-5680-5
Edition Number
1
Number of Pages
IX, 398
Number of Illustrations
49 b/w illustrations
Topics