Overview
- Provides comprehensive information on all key RNA-based techniques used for functional studies of small RNAs and genes
- Discusses recentmodifications and the latest trends concerning the main techniques, including cutting-edge methods
- Details various aspects of CRISPR technology, including DNA editing and beyond
- Elaborates on various applications and mode of usage of all techniques to be applied on various plant species for multiple purposes
Part of the book series: Concepts and Strategies in Plant Sciences (CSPS)
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Table of contents (17 chapters)
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Front Matter
Keywords
About this book
Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further developmentand applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing.
Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.
Editors and Affiliations
About the editors
Dr. Sachin Teotia is an associate professor in department of Biotechnology, Sharda University, India. He did his Ph.D. from Ohio State University, Columbus, USA. He did his postdoctoral work at Michigan Technological University, USA. He has an extensive training and experience of working in the field of plant developmental and stress biology using molecular and biochemical tools, including miRNAs and artificial miRNAs, as evident by the publications record. He worked on application of STTMs in Arabidopsis and rice for functional interrogation of many miRNAs. He also worked on two-hit amiRNA technology and its various applications. His current work focuses on functional genomics using amiRNA- and CRISPR/Cas-based modulation of gene expression controlling key traits in selected crop plants.
Dr. Xiaoqing Tang is an associate professor in department of Biological Sciences, Michigan Technological University, U.S.A. She did her Ph.D. from Weizmann Institute of Science, Israel, 2003. She has been involved in the characterization of miRNAs in control of insulin production and secretion in pancreatic beta cells. She successfully developed a high-throughput miRNA array technology for the study of miRNAs in human and mouse tissues. Her research involves identification and characterization of type-2 diabetes-associated miRNAs by high-throughput profiling of miRNA expressions using a diabetic mouse model. Furthermore, she is working to find miRNAs as diagnostic biomarkers for diabetes and develop novel miRNA drugs for the therapeutic treatment of diabetes.
Dr. Deepali Singh is an assistant professor in school of Biotechnology, Gautam Buddha University, India. She achieved her doctoral degree from Delhi University, India. Shedid her postdoctoral work at The Ohio State University, Columbus, USA. She got trained in studying RNA-protein interactions of RNase P. She also studied RNA structure of the retroviral post transcriptional element and its role in regulating viral transcription and translation. She has an extensive training and experience of about two decades of working in plant molecular biology and biotechnology. Her current research interests lie in the field of genomics and genome engineering for studying plant-pathogen interactions.
Bibliographic Information
Book Title: RNA-Based Technologies for Functional Genomics in Plants
Editors: Guiliang Tang, Sachin Teotia, Xiaoqing Tang, Deepali Singh
Series Title: Concepts and Strategies in Plant Sciences
DOI: https://doi.org/10.1007/978-3-030-64994-4
Publisher: Springer Cham
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Nature Switzerland AG 2021
Hardcover ISBN: 978-3-030-64993-7Published: 12 April 2021
Softcover ISBN: 978-3-030-64996-8Published: 12 April 2022
eBook ISBN: 978-3-030-64994-4Published: 11 April 2021
Series ISSN: 2662-3188
Series E-ISSN: 2662-3196
Edition Number: 1
Number of Pages: XIII, 428
Number of Illustrations: 4 b/w illustrations, 44 illustrations in colour
Topics: Plant Sciences, Plant Genetics and Genomics, Physiology