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Bioanalytical Reviews

Label-Free Monitoring of Cells in vitro

Editors: Wegener, Joachim (Ed.)

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eBook $219.00
price for USA in USD (gross)
  • ISBN 978-3-030-32433-9
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Hardcover $279.99
price for USA in USD
  • ISBN 978-3-030-32432-2
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  • Usually dispatched within 3 to 5 business days.
About this book

This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications.

Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.


Table of contents (7 chapters)

Table of contents (7 chapters)
  • Impedance-Based Assays Along the Life Span of Adherent Mammalian Cells In Vitro: From Initial Adhesion to Cell Death

    Pages 1-75

    Stolwijk, Judith A. (et al.)

  • Transistor-Based Impedimetric Monitoring of Single Cells

    Pages 77-110

    Hempel, F. (et al.)

  • Label-Free Monitoring of 3D Tissue Models via Electrical Impedance Spectroscopy

    Pages 111-134

    Alexander, Frank, Jr. (et al.)

  • On the Use of the Quartz Crystal Microbalance for Whole-Cell-Based Biosensing

    Pages 135-162

    Johannsmann, D.

  • Microphysiometry

    Pages 163-188

    Brischwein, Martin (et al.)

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-3-030-32433-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-3-030-32432-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Label-Free Monitoring of Cells in vitro
Editors
  • Joachim Wegener
Series Title
Bioanalytical Reviews
Series Volume
2
Copyright
2019
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-030-32433-9
DOI
10.1007/978-3-030-32433-9
Hardcover ISBN
978-3-030-32432-2
Series ISSN
1867-2086
Edition Number
1
Number of Pages
XI, 277
Number of Illustrations
80 illustrations in colour
Topics