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Advances in Biochemical Engineering/Biotechnology

Catalytically Active Nucleic Acids

Editors: Seitz, Harald, Stahl, Frank, Walter, Johanna-Gabriela (Eds.)

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  • Highlights fundamental aspects of nucleic acids enzymes
  • Addresses methods and applications
  • Discusses challenges in and prospects for nucleic acid enzyme engineering
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eBook $149.00
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  • ISBN 978-3-030-29646-9
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Hardcover $279.99
price for USA in USD
  • ISBN 978-3-030-29645-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume reviews numerous reaction mechanisms and applications of nucleic acids with catalytic activity. Written by an interdisciplinary team of authors, it provides an essential overview of these acids’ fundamental aspects, while also addressing associated methodologies such as nucleic acid enzyme engineering, peroxidase-mimicking DNAzymes and Aptazymes.

After the discovery of natural ribozymes – RNA molecules that mediate the cleavage and formation of phosphodiester bonds and the formation of peptide bonds – numerous artificial ribozymes with altered catalytic activities were produced by in vitro and in vivo selection. Unlike ribozymes, DNAzymes do not occur in nature. Although the catalytic activity of nucleic acid enzymes is usually much slower than that of proteins, nucleic acid enzymes with comparable catalytic activity have been obtained using stringent selection processes. The key advantages of these enzymes: they are e.g. smaller, easier to produce and purify than proteins, and can withstand denaturation, e.g. by heat.

Over the last few years, the number of publications on the applications of enzymatic nucleic acids has grown steadily. Summarizing the fundamentals and applications of these acids, this book will not only be an excellent resource for experts in the field but will also guide young researchers just starting out in this significant area.

About the authors

Harald Seitz has been a group leader at the Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses in Potsdam-Golm since 2012. He received his PhD (2000) and Diploma (1997) from the Free University Berlin and his postdoctoral degree (Habilitation (2012) from the University of Kassel. He worked for more than 10 years at Max-Planck Institute for Molecular Genetics in Berlin and moved in 2012 to the Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses. His work chiefly focuses on the (bio)analysis of different samples such as human serum or environmental samples using a variety of methods.

Table of contents (6 chapters)

Table of contents (6 chapters)
  • RNA G-Quadruplexes as Key Motifs of the Transcriptome

    Pages 1-20

    Rouleau, Samuel (et al.)

  • Challenges and Perspectives in Nucleic Acid Enzyme Engineering

    Pages 21-35

    Balke, Darko (et al.)

  • Strategies for Characterization of Enzymatic Nucleic Acids

    Pages 37-58

    Javadi-Zarnaghi, Fatemeh (et al.)

  • Bioanalytical Application of Peroxidase-Mimicking DNAzymes: Status and Challenges

    Pages 59-84

    Kosman, J. (et al.)

  • Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme: Principle and Biosensing Application

    Pages 85-106

    Alizadeh, Negar (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-3-030-29646-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-3-030-29645-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Catalytically Active Nucleic Acids
Editors
  • Harald Seitz
  • Frank Stahl
  • Johanna-Gabriela Walter
Series Title
Advances in Biochemical Engineering/Biotechnology
Series Volume
170
Copyright
2020
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-030-29646-9
DOI
10.1007/978-3-030-29646-9
Hardcover ISBN
978-3-030-29645-2
Series ISSN
0724-6145
Edition Number
1
Number of Pages
VII, 123
Number of Illustrations
26 b/w illustrations, 12 illustrations in colour
Topics