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Carbonic Anhydrase as Drug Target

Thermodynamics and Structure of Inhibitor Binding

Editors: Matulis, Daumantas (Ed.)

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  • ​Numerous X-ray crystallographic structures of human carbonic anhydrases and their complexes with inhibitors  
  • Compares methods to determine inhibitor binding  
  • Provides information on intrinsic thermodynamics of inhibitor binding to carbonic anhydrases
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-3-030-12780-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-3-030-12778-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book offers deep insights into the thermodynamics and molecular structures of the twelve catalytically active isoforms of human carbonic anhydrase (CA) with a particular focus on inhibitor binding for drug design. X-ray crystallographic structures in combination with enzyme kinetic testing provide information on the interaction of CAs and their inhibitors, knowledge which is crucial for rational drug design.   CAs are zinc carrying enzymes that catalyse the reversible interconversion of carbon dioxide and bicarbonate and are involved in numerous cellular processes. They are therefore a common target for drugs. The suppression of CA activities through inhibitory compounds has found application for example in diuretics and in glaucoma therapy. In this book methods used to determine binding thermodynamics of inhibitory compounds (Isothermal titration calorimetry, Fluorescent thermal shift assay/differential scanning fluorimetry and others) will be compared in detail. Also types and chemical synthesis of CA inhibitors, the use of antibodies against CAs as well as inhibitor application in animals are discussed.  

About the authors

Prof. Dr. Daumantas Matulis

Vilnius University

Life Sciences Center

Institute of Biotechnology

Department of Biothermodynamics and Drug Design

Sauletekio 7, 10257 Vilnius

Lithuania

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Overview of Human Carbonic Anhydrases

    Pages 3-14

    Baranauskienė, Lina (et al.)

  • Recombinant Production of 12 Catalytically Active Human CA Isoforms

    Pages 15-37

    Mickevičiūtė, Aurelija (et al.)

  • Catalytic Activity and Inhibition of Human Carbonic Anhydrases

    Pages 39-49

    Baranauskienė, Lina (et al.)

  • Characterization of Carbonic Anhydrase Thermal Stability

    Pages 51-59

    Zubrienė, Asta (et al.)

  • Inhibitor Binding to Carbonic Anhydrases by Fluorescent Thermal Shift Assay

    Pages 63-78

    Petrauskas, Vytautas (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-3-030-12780-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-3-030-12778-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Carbonic Anhydrase as Drug Target
Book Subtitle
Thermodynamics and Structure of Inhibitor Binding
Editors
  • Daumantas Matulis
Copyright
2019
Publisher
Springer International Publishing
Copyright Holder
Springer Nature Switzerland AG
eBook ISBN
978-3-030-12780-0
DOI
10.1007/978-3-030-12780-0
Hardcover ISBN
978-3-030-12778-7
Edition Number
1
Number of Pages
XXVI, 353
Number of Illustrations
62 b/w illustrations, 107 illustrations in colour
Topics