Overview
- Editors:
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Andrew K. Ottens
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Dept. Anatomy & Neurobiology, Virginia Commonwealth University School of Medicine, Richmond, U.S.A.
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Kevin K.W. Wang
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Center of Innovative Research, Banyan Biomarkers, Inc. Center of Innovative Research, Alachua, U.S.A.
- Presents a modular approach to experimental design, covering neurodegenerative models, subproteome selection technologies, quantitative and qualitative mass spectrometry, data analysis and clinical translation of results
- Detailed guide to developing, running and analyzing broadly applicable neuroproteomic experiments
- Provides specifics and insight to perform the latest quantitative and subproteome analyses, including key post-translational modifications
- Covers a wide range of model systems used in neuroproteomic research, including ischemia, brain and spinal cord injury, substance abuse and neurodegenerative disease
- Expands beyond the lab bench in providing guidance on translating neuroproteomic results to the clinic
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Table of contents (20 protocols)
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Disease Models in Neuroproteomics
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- Jitendra R. Dave, Anthony J. Williams, Changping Yao, X.-C. May Lu, Frank C. Tortella
Pages 25-40
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- András Büki, Erzsébet Kövesdi, József Pál, Endre Czeiter
Pages 41-55
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- Anshu Chen, Joe E. Springer
Pages 57-67
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- Scott E. Hemby, Nilesh Tannu
Pages 69-83
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- Andrew T. N. Tebbenkamp, David R. Borchelt
Pages 85-91
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Sub-Proteome Separations and Neuroproteomic Analysis
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- Stefani N. Thomas, Diane Cripps, Austin J. Yang
Pages 109-121
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- Rukhsana Sultana, D. Allan Butterfield
Pages 123-135
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- Xianquan Zhan, Dominic M. Desiderio
Pages 137-163
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- Kurt Krapfenbauer, Michael Fountoulakis
Pages 165-180
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- Zhiqun Zhang, Firas H. Kobeissy, Andrew K. Ottens, Juan A. Martínez, Kevin K. W. Wang
Pages 181-190
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Neuroproteomic Methodology and Bioinformatics
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Front Matter
Pages 191-191
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- Brian F. Fuller, Andrew K. Ottens
Pages 193-200
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- Tong Liu, Jun Hu, Hong Li
Pages 201-216
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- Firas H. Kobeissy, Zhiqun Zhang, Shankar Sadasivan, Mark S. Gold, Kevin K. W. Wang
Pages 217-228
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- Bingwen Lu, Tao Xu, Sung Kyu Park, Daniel B. McClatchy, Lujian Liao, John R. Yates III
Pages 229-259
About this book
Neuroproteomics: Methods and Protocols presents experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. The target audience includes clinical or basic scientists who look to apply proteomics to the neurosciences. Often researchers hear of proteomics without an adequate explanation of the methodology and inherent limitations. This volume conveys where proteomic methodology is in its application to CNS research and what results can be expected. We also address clinical translation of neuroproteomics, specifically in the area of biomarker research. The inception of neuroproteomics capitalized on rapid progress in large-molecule mass spectrometry over the last decade. Two seminal advances have spurred research – development of reliable polypeptide ionization processes and bioinformatics to rapidly process tandem mass spectra for peptide identification and quantification. What has followed is the exponential application of mass spectrometry to proteome characteri- tion across biological and biomedical disciplines. Arguably, the most elaborate proteomic implementation is in studying the CNS, the most enigmatic and complex animal system. Neuroscience is characterized by grandiose questions – what is consciousness, how does thought or memory work. Neuroproteomics researchers, however, have pri- rily involved themselves dysfunction, based on a pressing need (and invariably funding), in answering questions on CNS dysfunction, based on a pressing need (and invariably funding), and because such questions hold more accessible answers. Dysfunction is readily contrasted against normal function and presumably produces a lasting differential protein signature.
Reviews
From the reviews:
“This book provides experimental details about animal models for neuroproteomic research, methods for separating and analyzing discrete subcomponents of the neuroproteome, and CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. … The authors intend the book for basic and clinical researchers, and it will be of much interest to basic scientists, clinical scientists, and students interested in applying proteomics to the neurosciences.” (Omer Iqbal, Doody’s Review Service, March, 2010)
Editors and Affiliations
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Dept. Anatomy & Neurobiology, Virginia Commonwealth University School of Medicine, Richmond, U.S.A.
Andrew K. Ottens
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Center of Innovative Research, Banyan Biomarkers, Inc. Center of Innovative Research, Alachua, U.S.A.
Kevin K.W. Wang
