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Methods in Molecular Biology

SUMO Protocols

Editors: Ulrich, Helle (Ed.)

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  • First methods collection devoted entirely to the SUMO system
  • Provides comprehensive practical advice from leading experts in the field
  • Covers a range of complementary approaches for SUMO target identification
  • Presents step-by-step protocols for the purification and manipulation of SUMO conjugation and de-conjugation enzymes
  • Includes a description of SUMO fusion technology for improved recombinant protein production
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eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-566-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
About this book

While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls.


Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.

Reviews

From the reviews:

"The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques … . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field … and regulated by SUMO. … for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves." (Peter O’Hare, Microbiology Today, July, 2009)


Table of contents (20 chapters)

Table of contents (20 chapters)
  • The SUMO System: An Overview

    Pages 3-16

    Ulrich, Helle D.

  • Identification of SUMO Target Proteins by Quantitative Proteomics

    Pages 19-31

    Andersen, Jens S. (et al.)

  • Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry

    Pages 33-49

    Wohlschlegel, James A.

  • Identification of SUMO Targets Through In Vitro Expression Cloning

    Pages 51-61

    Gocke, Christian B. (et al.)

  • Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)

    Pages 63-79

    Niedenthal, Rainer

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-566-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
SUMO Protocols
Editors
  • Helle Ulrich
Series Title
Methods in Molecular Biology
Series Volume
497
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-566-4
DOI
10.1007/978-1-59745-566-4
Hardcover ISBN
978-1-934115-80-0
Softcover ISBN
978-1-61737-945-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 324
Number of Illustrations
70 b/w illustrations
Topics