- First methods collection devoted entirely to the SUMO system
- Provides comprehensive practical advice from leading experts in the field
- Covers a range of complementary approaches for SUMO target identification
- Presents step-by-step protocols for the purification and manipulation of SUMO conjugation and de-conjugation enzymes
- Includes a description of SUMO fusion technology for improved recombinant protein production
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- About this book
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While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls.
Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system. - Reviews
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From the reviews:
"The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques … . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field … and regulated by SUMO. … for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves." (Peter O’Hare, Microbiology Today, July, 2009)
- Table of contents (20 chapters)
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The SUMO System: An Overview
Pages 3-16
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Identification of SUMO Target Proteins by Quantitative Proteomics
Pages 19-31
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Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry
Pages 33-49
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Identification of SUMO Targets Through In Vitro Expression Cloning
Pages 51-61
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Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)
Pages 63-79
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- SUMO Protocols
- Editors
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- Helle Ulrich
- Series Title
- Methods in Molecular Biology
- Series Volume
- 497
- Copyright
- 2009
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59745-566-4
- DOI
- 10.1007/978-1-59745-566-4
- Hardcover ISBN
- 978-1-934115-80-0
- Softcover ISBN
- 978-1-61737-945-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 324
- Number of Illustrations
- 70 b/w illustrations
- Topics
