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Methods in Molecular Biology

Protein Blotting and Detection

Methods and Protocols

Editors: Kurien, Biji T., Scofield, R. Hal (Eds.)

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  • Includes 52 different protein transfer and detection techniques
  • Gives a brief overview of 35 additional protein transfer and detection methodologies
  • Provides a history of protein transfer development and an overview of membrane proteomics
  • Outlines inexpensive protein blotting and detection protocols useful for large college courses
  • Allows investigators to rapidly implement a technique that is new to them and to their laboratories
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eBook $149.00
price for USA in USD
  • ISBN 978-1-59745-542-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
Softcover $199.00
price for USA in USD
About this book

Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.

Reviews

From the reviews:

"This informative, hands-on-guide is essential for all investigators interested in learning techniques based on Western blotting which they can easily transfer to their laboratories. … The book is intended for any investigator interested in learning a new technique based on Western blotting. … this hands-on guide will be quite useful and helpful for both investigators and students working in these areas." (Omer Iqbal, Doody’s Review Service, October, 2009)


Table of contents (57 chapters)

Table of contents (57 chapters)
  • Origins of Protein Blotting

    Pages 1-3

    Towbin, Harry

  • Western Blotting: Remembrance of Things Past

    Pages 5-8

    Burnette, W. Neal

  • Introduction to Protein Blotting

    Pages 9-22

    Kurien, Biji T. (et al.)

  • Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis

    Pages 23-33

    Lee, Der-Yen (et al.)

  • Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support

    Pages 35-38

    Olsen, Ingrid (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-59745-542-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
Softcover $199.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Protein Blotting and Detection
Book Subtitle
Methods and Protocols
Editors
  • Biji T. Kurien
  • R. Hal Scofield
Series Title
Methods in Molecular Biology
Series Volume
536
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-542-8
DOI
10.1007/978-1-59745-542-8
Hardcover ISBN
978-1-934115-73-2
Softcover ISBN
978-1-61737-943-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 588
Number of Illustrations
135 b/w illustrations, 6 illustrations in colour
Topics