Methods in Molecular Biology

Potassium Channels

Methods and Protocols

Editors: Lippiat, Jonathan D. (Ed.)

  • Contains detailed protocols that describe the use of routine and advanced electrophysiological techniques, such as whole cell patch clamp, perforated patch clamp, excised macropatch, open-cell cell-attached patch clamp, planar patch clamp, and artificial bilayer
  • Of major pharmacological interest as a number of chapters describe the use of potassium channel drug screening platforms, planar electrophysiology, and the cardiac safety pharmacology of HERG potassium channels
  • Describes optical techniques that allow the real-time study of protein interactions and conformational changes in potassium channels
  • Examines both functional experiments and protocols that allow the evaluation of the cellular biology of potassium channels, like transcription, splicing, and protein trafficking
  • Features standard laboratory techniques such as cell culture and transfection, oocyte injection, Western blotting, PCR to support these experiments
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59745-526-8
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Hardcover $179.00
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  • ISBN 978-1-934115-65-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-940-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.

Reviews

From the reviews:

"This is a very informative book, covering a variety of methods available to study potassium channels … . The protocols are clearly organized and usefully detailed. … Illustrative figures and in some cases, photographs of equipment … adding to the clarity of the methods sections. This book … a valuable reference not only for those already in the potassium channel field but also for studies of other ion channel systems. … each chapter allows bench scientists to undertake new, informed, experimental approaches in their research." (Samantha Miller, Microbiology Today, July, 2009)

“This book is dedicated to experimental methods, which allow us to investigate potassium channels from their genes through gene transcription to protein structure, function and regulation in health and disease. … chapters in this book are written in form of practical laboratory protocols. Therefore the book will be primarily used by practicing scientists and by graduated and post-graduate students for their practical research. Certainly it will find its place in many laboratories investigating various features of potassium channels regulations.” (Ľubica Lacinová, General Physiology and Biophysics, Vol. 29, April, 2010)

Table of contents (22 chapters)

  • Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA—Protein Interactions by Chromatin Immunoprecipitation

    Ooi, Lezanne (et al.)

    Pages 3-17

  • Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries

    Cheong, Alex (et al.)

    Pages 19-33

  • Cloning of Potassium Channel Splice Variants from Tissues and Cells

    Chen, Lie (et al.)

    Pages 35-60

  • Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels

    Smith, Andrew J. (et al.)

    Pages 63-68

  • Investigation of KATP Channel Endocytosis by Immunofluorescence

    Smith, Andrew J. (et al.)

    Pages 69-77

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59745-526-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-934115-65-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-940-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Potassium Channels
Book Subtitle
Methods and Protocols
Editors
  • Jonathan D. Lippiat
Series Title
Methods in Molecular Biology
Series Volume
491
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-526-8
DOI
10.1007/978-1-59745-526-8
Hardcover ISBN
978-1-934115-65-7
Softcover ISBN
978-1-61737-940-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 302
Number of Illustrations and Tables
59 b/w illustrations
Topics