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Methods in Molecular Biology

Auditory and Vestibular Research

Methods and Protocols

Editors: Sokolowski, Bernd (Ed.)

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  • Lists all the reagents, step-by-step techniques, and limitations for reproducible results in DNA/RNA tracing, protein identification, and microscopic imaging
  • Clearly describes the protocols necessary for in vivo and in vitro gene delivery techniques
  • Provides the tools to identify and isolate cochlear stem cells for the treatment of inner ear pathologies
  • Includes lineage and fate mapping techniques for peripheral and central studies of auditory/vestibular development
  • Describes techniques for identification and verification of protein-protein interactions
  • Provides important tools for the basic and clinical researcher to identify genes and microbes underlying diseases of the ear including the cloning of deafness genes
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eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-523-7
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Hardcover $179.00
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About this book

While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning

    Pages 3-20

    Trujillo-Provencio, Casilda (et al.)

  • Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays

    Pages 21-29

    Vázquez, Ana E. (et al.)

  • In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections

    Pages 31-46

    Hiel, Hakim

  • Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification

    Pages 47-64

    Satoh, Takunori (et al.)

  • Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus

    Pages 65-85

    Kim, Jun Chul (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59745-523-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Auditory and Vestibular Research
Book Subtitle
Methods and Protocols
Editors
  • Bernd Sokolowski
Series Title
Methods in Molecular Biology
Series Volume
493
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-523-7
DOI
10.1007/978-1-59745-523-7
Hardcover ISBN
978-1-934115-62-6
Softcover ISBN
978-1-61737-939-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 428
Number of Illustrations
48 b/w illustrations, 13 illustrations in colour
Topics

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