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Chloroplast Biotechnology

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Pal Maliga

    1. Rutgers University, Waksman Inst.of Microbiology, Piscataway, USA

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  • Presents current up-to-date methodologies on chloroplast molecular biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1132)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Background

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Plastid Genomes of Flowering Plants

      • Tracey A. Ruhlman, Robert K. Jansen
      Pages 3-38

    3. Next-Generation Technologies to Determine Plastid Genome Sequences

      • Robert J. Henry, Nicole Rice, Mark Edwards, Catherine J. Nock
      Pages 39-46

    4. Plastid Gene Transcription: Promoters and RNA Polymerases

      • Jennifer Ortelt, Gerhard Link
      Pages 47-72

    5. Plastid mRNA Translation

      • Masahiro Sugiura
      Pages 73-91

    6. Engineering Chloroplasts for High-Level Foreign Protein Expression

      • Ralph Bock
      Pages 93-106

    7. Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences

      • Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila, Anil Day
      Pages 107-123

    8. Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-green Plastids

      • Maureen R. Hanson, Amirali Sattarzadeh
      Pages 125-143

  3. Nicotiana tabacum the Model Species of Chloroplast Biotechnology

    1. Front Matter

      Pages 145-145
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  4. Nicotiana tabacum the Model Species of Chloroplast Biotechnology

    1. Plastid Transformation in Nicotiana tabacum and Nicotiana sylvestris by Biolistic DNA Delivery to Leaves

      • Pal Maliga, Tarinee Tungsuchat-Huang
      Pages 147-163

    2. Nicotiana tabacum : PEG-Mediated Plastid Transformation

      • Areli Herrera Díaz, Hans-Ulrich Koop
      Pages 165-175

    3. Plastid Transformation of Tobacco Suspension Cell Cultures

      • Jeffrey M. Staub
      Pages 177-185

    4. Tryptophan and Indole Analog Mediated Plastid Transformation

      • Pierluigi Barone, Xing-Hai Zhang, Jack M. Widholm
      Pages 187-203

    5. Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre Recombinase

      • Tarinee Tungsuchat-Huang, Pal Maliga
      Pages 205-220

    6. Determination of the Half-Life of Chloroplast Transcripts in Tobacco Leaves

      • Sithichoke Tangphatsornruang, John C. Gray
      Pages 221-234

    7. Quantification of Organellar DNA and RNA Using Real-Time PCR

      • Andreas Weihe
      Pages 235-243

    8. Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expression

      • Spencer M. Whitney, Robert E. Sharwood
      Pages 245-262

  5. Crop Specific Plastid Transformation Protocols

    1. Front Matter

      Pages 263-263
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    2. Plastid Transformation in Tomato

      • Stephanie Ruf, Ralph Bock
      Pages 265-276
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Keywords

About this book

In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoidance of known pitfalls.

Authoritative and practical,  Chloroplast Biotechnology: Methods and Protocols seek to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology and healthcare.

Editors and Affiliations

  • Rutgers University, Waksman Inst.of Microbiology, Piscataway, USA

    Pal Maliga

Bibliographic Information

  • Book Title: Chloroplast Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Pal Maliga

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-995-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-994-9Published: 05 March 2014

  • Softcover ISBN: 978-1-4939-5870-2Published: 03 September 2016

  • eBook ISBN: 978-1-62703-995-6Published: 08 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 452

  • Number of Illustrations: 42 b/w illustrations, 48 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

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