Human Cytomegaloviruses
Methods and Protocols
Editors: Yurochko, Andrew D., Miller, William E. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.
- Table of contents (18 chapters)
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History of the Molecular Biology of Cytomegaloviruses
Pages 1-14
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Overview of Human Cytomegalovirus Pathogenesis
Pages 15-28
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Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
Pages 29-46
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Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection
Pages 47-57
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Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants
Pages 59-79
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Table of contents (18 chapters)
- Download Preface 1 PDF (93.5 KB)
- Download Sample pages 1 PDF (242.2 KB)
- Download Table of contents PDF (111 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Human Cytomegaloviruses
- Book Subtitle
- Methods and Protocols
- Editors
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- Andrew D. Yurochko
- William E. Miller
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1119
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer New York
- eBook ISBN
- 978-1-62703-788-4
- DOI
- 10.1007/978-1-62703-788-4
- Hardcover ISBN
- 978-1-62703-787-7
- Softcover ISBN
- 978-1-4939-6273-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 368
- Number of Illustrations
- 18 b/w illustrations, 22 illustrations in colour
- Topics
