Overview
- Presents introductory material, methods to investigate changes in neuronal and glial activity, and optical imaging methods probing metabolic states
- Features detailed techniques from the labs of experts
- Includes key implementation advice
- Includes supplementary material: sn.pub/extras
Part of the book series: Neuromethods (NM, volume 85)
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Table of contents (16 protocols)
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Front Matter
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Fundamentals of Using Light to Study Neocortical Dynamics
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Front Matter
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Shedding Light on Cortical Activity States
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Front Matter
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Elucidating Metabolic States of Cortical Circuits
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Front Matter
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Keywords
About this book
The application of optical methods for investigating neocortical circuit dynamics has greatly expanded in recent years, providing novel insights into the fascinating world of brain function. Optical Imaging of Neocortical Dynamics presents a guide to these indispensible tools, which cover a broad range of spatiotemporal scales and a large variety of signaling aspects, ranging from voltage changes to metabolic states. This detailed volume specifically explores methods that are applied in experiments on living brains (in vivo) and that relate to functional properties on the spatial scale of cortical circuits. Beginning with an introductory section that focuses on physical fundamentals of optical imaging as well as molecular tools used for in vivo optical imaging and optogenetic control, the book continues with the most relevant methods and their applications to investigate changes in neuronal and glial activity states as well as optical imaging methods probing metabolic states. Written for the Neuromethods series, this volume contains the kind of detail and key implementation advice that ensures successful results in the lab.
Practical and easy to use, Optical Imaging of Neocortical Dynamics serves as an ideal guide for researchers who aim to apply these highly valuable tools to their own neuroscientific studies.
Editors and Affiliations
Bibliographic Information
Book Title: Optical Imaging of Neocortical Dynamics
Editors: Bruno Weber, Fritjof Helmchen
Series Title: Neuromethods
DOI: https://doi.org/10.1007/978-1-62703-785-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Science+Business Media New York 2014
Hardcover ISBN: 978-1-62703-784-6Published: 24 January 2014
Softcover ISBN: 978-1-4939-5970-9Published: 24 September 2016
eBook ISBN: 978-1-62703-785-3Published: 23 January 2014
Series ISSN: 0893-2336
Series E-ISSN: 1940-6045
Edition Number: 1
Number of Pages: XII, 296
Number of Illustrations: 3 b/w illustrations, 70 illustrations in colour
Topics: Neurosciences, Neurology