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Methods in Molecular Biology

Electron Microscopy

Methods and Protocols

Editors: Kuo, John (Ed.)

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  • Deals with biological and molecular research techniques based on TEM and SEM
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-62703-776-1
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Hardcover $299.99
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Softcover $219.99
price for USA in USD
  • ISBN 978-1-4939-6073-6
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  • Immediate ebook access, if available*, with your print order
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About this book

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

Reviews

From the book reviews:

“The amount of information in this book is impressive. … The layout of the book is well-organized and the protocols with their accompanying notes are particularly useful and comprehensive. … I would highly recommend this book to any EM laboratory that does not already have the second edition and to larger EM research facilities that are performing the latest correlative microscopy and image analysis techniques.” (Kath White, Microscopy and Microanalysis, Vol. 20 (5), October, 2014)

Table of contents (34 chapters)

Table of contents (34 chapters)
  • Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells

    Pages 1-19

    Bozzola, John J.

  • Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy

    Pages 21-37

    Webster, Paul

  • Processing Plant Tissues for Ultrastructural Study

    Pages 39-55

    Kuo, John

  • Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains

    Pages 57-72

    Ellis, E. Ann

  • Metal Shadowing for Electron Microscopy

    Pages 73-93

    Hendricks, Gregory M.

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-62703-776-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $299.99
price for USA in USD
  • ISBN 978-1-62703-775-4
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • ISBN 978-1-4939-6073-6
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Electron Microscopy
Book Subtitle
Methods and Protocols
Editors
  • John Kuo
Series Title
Methods in Molecular Biology
Series Volume
1117
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-62703-776-1
DOI
10.1007/978-1-62703-776-1
Hardcover ISBN
978-1-62703-775-4
Softcover ISBN
978-1-4939-6073-6
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XVIII, 799
Number of Illustrations
156 b/w illustrations, 100 illustrations in colour
Topics

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