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Methods in Molecular Biology

Gene Correction

Methods and Protocols

Editors: Storici, Francesca (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-62703-761-7
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About this book

Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.

Table of contents (29 chapters)

Table of contents (29 chapters)
  • RecTEPsy-Mediated Recombineering in Pseudomonas syringae

    Pages 3-10

    Swingle, Bryan

  • Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila

    Pages 11-24

    Zhang, Yi (et al.)

  • Multiple Genetic Manipulations of DT40 Cell Line

    Pages 25-35

    Motegi, Akira (et al.)

  • Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination

    Pages 37-55

    Howden, Sara E. (et al.)

  • Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells

    Pages 59-83

    Bertoni, Carmen

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-62703-761-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-62703-760-0
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-6321-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Gene Correction
Book Subtitle
Methods and Protocols
Editors
  • Francesca Storici
Series Title
Methods in Molecular Biology
Series Volume
1114
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-761-7
DOI
10.1007/978-1-62703-761-7
Hardcover ISBN
978-1-62703-760-0
Softcover ISBN
978-1-4939-6321-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 463
Number of Illustrations
28 b/w illustrations, 59 illustrations in colour
Topics

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