Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1104)
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Table of contents (26 protocols)
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Front Matter
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Cell Line Development and Selection
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Front Matter
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Techniques for Process Development
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Front Matter
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Cell Characterisation and Analysis
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Front Matter
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Keywords
About this book
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Editors and Affiliations
Bibliographic Information
Book Title: Animal Cell Biotechnology
Book Subtitle: Methods and Protocols
Editors: Ralf Pörtner
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-733-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-732-7Published: 04 December 2013
Softcover ISBN: 978-1-4939-6318-8Published: 23 August 2016
eBook ISBN: 978-1-62703-733-4Published: 03 December 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIV, 498
Number of Illustrations: 55 b/w illustrations, 28 illustrations in colour
Topics: Biotechnology, Cell Culture